caa a22 4500 465811930 CHVBK 20180323112118.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF02377311 doi (NATIONALLICENCE)springer-10.1007/BF02377311 A possible role of bone morphogenetic protein (BMP) in the regulation of bone remodeling [Elektronische Daten] [Hideki Yoshikawa, Kunio Takaoka] Bone morphogenetic protein (BMP) from an osteosarcoma powder was found to be solubilized in weak acid (pH 2.6-3.0), but not in acidic solutions of a lower or higher pH. This indicates that the solubility of BMP strictly depends on the hydrogen ion concentration of the solution. Ectopic osteogenesis induced by the osteosarcoma powder was greatly enhanced by treating the powder with acid solutions below pH 3.0. The acid-treated preparations were neutralized before bioassay. Acid treatment resulted in almost a five-fold increase in the amount of new bone formation. This finding suggests that a high concentration of hydrogen ion is important in regulating the activity of BMP. Lyophilized cultured cells of the osteosarcoma preserved the osteogenic activity even after incubation in acidic and neutral buffers at 37°C for six days. This observation suggests that BMP is stable to endogenous enzymes such as lysosomal or proteolytic enzymes. Based on these results, the role possibly played by BMP in the regulation of bone remodeling is discussed. Japanese Society of Bone Metabolism Research, 1990 Bone remodeling nationallicence Bone morphogenetic protein nationallicence Ectopic bone nationallicence Osteoclast nationallicence Acid solution nationallicence Yoshikawa Hideki Department of Orthopaedic Surgery, Osaka University Medical School, 1-1-50, Fukushima-ku, 553, Osaka, Japan aut Takaoka Kunio Department of Orthopaedic Surgery, Osaka University Medical School, 1-1-50, Fukushima-ku, 553, Osaka, Japan aut Journal of Bone and Mineral Metabolism Springer-Verlag 8/2(1990-08-01), 34-40 0914-8779 8:2<34 1990 8 774 https://doi.org/10.1007/BF02377311 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02377311 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yoshikawa Hideki Department of Orthopaedic Surgery, Osaka University Medical School, 1-1-50, Fukushima-ku, 553, Osaka, Japan aut NATIONALLICENCE 700 1- Takaoka Kunio Department of Orthopaedic Surgery, Osaka University Medical School, 1-1-50, Fukushima-ku, 553, Osaka, Japan aut NATIONALLICENCE 773 0- Journal of Bone and Mineral Metabolism Springer-Verlag 8/2(1990-08-01), 34-40 0914-8779 8:2<34 1990 8 774 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer