caa a22 4500 465820263 CHVBK 20180323112133.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF01650475 doi (NATIONALLICENCE)springer-10.1007/BF01650475 Autonomous growth of lymphoid cells following IL-2 expression from retrovirus vectors containing HIV-1 trans -acting elements [Elektronische Daten] [Ilya Shapiro, Craig Meier, Victoria Vlach, Thomas McDonald, Hans Wigzell, Mario Stevenson] The human immunodeficiency virus type I (HIV-1) possesses powerful regulatory elements that control the rate of replication of HIV-1 and subsequent processing ofHIV-1 genes. We have used this regulatory mechanism to drive expression of foreign genes inserted in retrovirus vectors. This approach was used to express the humanIL-2 gene in IL-2-dependent mouse CTLL-2 cells to determine the role of autonomous growth in maintaining proliferation of virus-infected T lymphocytes during HTLV-1-induced adult T-cell leukemia (ATL). Expression of IL-2 sequences in IL-2-dependent mouse CTLL-2 cells resulted in autonomous growth of IL-2-independent CTLL-2 clones. Endogenous expression of IL-2 appeared to interrupt normal constraints of growth in that these IL-2-independent clones showed reduced cell-density-dependent inhibition but not a tumorigenic phenotype. IL-2-independent CTLL-2 clones did not secrete detectable quantities of IL-2 into culture supernatant and exhibited reduced sensitivity to the inhibitory effects of both IL-2 and IL-2 receptor antibody. These results suggest that the IL-2 autocrine loop within these cells involves intracellular IL-2/IL-2 receptor binding. The apparent lack of IL-2 production and poor responsiveness to IL-2 or IL-2 antibodies displayed by cell lines from ATL patients may be explained by an intracellular IL-2/IL-2 receptor autocrine loop. Plenum Publishing Corporation, 1990 Shapiro Ilya Department of Immunology, Karolinska Institute, Stockholm, Sweden aut Meier Craig Molecular Biology Laboratory, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska aut Vlach Victoria Molecular Biology Laboratory, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska aut McDonald Thomas Molecular Biology Laboratory, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska aut Wigzell Hans Department of Immunology, Karolinska Institute, Stockholm, Sweden aut Stevenson Mario Molecular Biology Laboratory, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/1(1990-01-01), 1-14 0740-7750 16:1<1 1990 16 11188 https://doi.org/10.1007/BF01650475 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01650475 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shapiro Ilya Department of Immunology, Karolinska Institute, Stockholm, Sweden aut NATIONALLICENCE 700 1- Meier Craig Molecular Biology Laboratory, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska aut NATIONALLICENCE 700 1- Vlach Victoria Molecular Biology Laboratory, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska aut NATIONALLICENCE 700 1- McDonald Thomas Molecular Biology Laboratory, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska aut NATIONALLICENCE 700 1- Wigzell Hans Department of Immunology, Karolinska Institute, Stockholm, Sweden aut NATIONALLICENCE 700 1- Stevenson Mario Molecular Biology Laboratory, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/1(1990-01-01), 1-14 0740-7750 16:1<1 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer