caa a22 4500 465820271 CHVBK 20180323112133.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF01650477 doi (NATIONALLICENCE)springer-10.1007/BF01650477 SV40-mediated tumor selection and chromosome transfer to enrich for cystic fibrosis region [Elektronische Daten] [David Porteous, Julia Dorin, Maureen Wilkinson, Judy Fletcher, Elisabeth Emslie, Veronica van Heyningen] The somatic cell hybrid C121, with chromosome 7 as its sole human component, arose when mouse macrophages were immortalized by fusion with SV40-transformed human fibroblasts. The transforming SV40 genomes are integrated at 7q31-7q35. We show that hybrids with a reduced chromosome 7 component, but which retain markers linked to the cystic fibrosis locus, can be generated by direct in vivo tumor selection or following chromosome-mediated gene transfer and SV40-mediated cellular transformation. Our methods for chromosome fragmentation and fine-structure mapping can now be applied to the substantial number of SV40-transformed human cell lines, with independent chromosomal integration sites, already available. Our results also suggest that expression of human epidermal growth factor receptor augments the tumorigenic potential of the SV40-transformed C121 hybrid. Plenum Publishing Corporation, 1990 Porteous David MRC Human Genetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK aut Dorin Julia MRC Human Genetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK aut Wilkinson Maureen MRC Human Genetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK aut Fletcher Judy MRC Human Genetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK aut Emslie Elisabeth MRC Human Genetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK aut van Heyningen Veronica MRC Human Genetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/1(1990-01-01), 29-38 0740-7750 16:1<29 1990 16 11188 https://doi.org/10.1007/BF01650477 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01650477 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Porteous David MRC Human Genetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK aut NATIONALLICENCE 700 1- Dorin Julia MRC Human Genetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK aut NATIONALLICENCE 700 1- Wilkinson Maureen MRC Human Genetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK aut NATIONALLICENCE 700 1- Fletcher Judy MRC Human Genetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK aut NATIONALLICENCE 700 1- Emslie Elisabeth MRC Human Genetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK aut NATIONALLICENCE 700 1- van Heyningen Veronica MRC Human Genetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/1(1990-01-01), 29-38 0740-7750 16:1<29 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer