caa a22 4500 465820298 CHVBK 20180323112134.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF01650476 doi (NATIONALLICENCE)springer-10.1007/BF01650476 Susceptibility for N-ras -mediated transformation requires loss of tumor suppressor activity [Elektronische Daten] [David Krizman, B. Giovanella, Michael Tainsky] We have been using PA-1 human teratocarcinoma cells to study mechanisms by which oncogenes induce transformation. Tumorigenic PA-1 cells at passages greater than 100 (>P100) contain a spontaneously activatedN-ras oncogene, while earlier-passage preneoplastic cells contain only the germ-line protooncogene and are nontumorigenic. One preneoplastic cell clone of PA-1 cells can be transformed by introduction of the cloned PA-1N-ras in gene-transfer experiments, while another earlier-passage clonal cell line cannot be transformed. The goal of this investigation was to determine how human cells progress from resistance to susceptibility toras oncogene-induced transformation. Somatic cell hybridization experiments described in this report indicate that the resistance of the low-passage cells to transformation is a dominant trait suppressing transformation. Loss of chromosomes from hybrid segregants suggested that tumor suppressors exist on chromosomes 1, 4, and 11. Extended in vitro passaging of somatic cell hybrids also resulted in the loss of chromosomes. Chromosome 1 was lost in these populations of cells, implying that reduction of this chromosome may promote proliferation and not specifically affect tumor formation. Plenum Publishing Corporation, 1990 Krizman David Department of Tumor Biology, The University of Texas M.D. Anderson Cancer Center, Houston, Texas aut Giovanella B. St. Joseph Hospital Cancer Research Laboratory, Houston, Texas aut Tainsky Michael Department of Tumor Biology, The University of Texas M.D. Anderson Cancer Center, Houston, Texas aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/1(1990-01-01), 15-27 0740-7750 16:1<15 1990 16 11188 https://doi.org/10.1007/BF01650476 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01650476 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Krizman David Department of Tumor Biology, The University of Texas M.D. Anderson Cancer Center, Houston, Texas aut NATIONALLICENCE 700 1- Giovanella B. St. Joseph Hospital Cancer Research Laboratory, Houston, Texas aut NATIONALLICENCE 700 1- Tainsky Michael Department of Tumor Biology, The University of Texas M.D. Anderson Cancer Center, Houston, Texas aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/1(1990-01-01), 15-27 0740-7750 16:1<15 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer