caa a22 4500 46582031X CHVBK 20180323112134.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF01650482 doi (NATIONALLICENCE)springer-10.1007/BF01650482 Stability of DNA methylation of X-chromosome genes during aging [Elektronische Daten] [F. Pagani, D. Toniolo, C. Vergani] The stability of DNA methylation during aging was assessed in two groups of young (5-20 years old) and old (85-95 years old) women in DNA from blood leukocytes. Three X-linked genes were investigated. Two,G6PD andGdX, are located on Xq28, on the inactivated portion of the X chromosome: demethylation of specific regions of both genes was shown previously to be directly correlated with gene reactivation. The third,MIC2, is located on the pseudoautosomal region of the X chromosome and escapes X inactivation. The 5′ region of theG6PD andGdX genes and the body of theG6PD, GDX, andMIC2 genes were analyzed with specific DNA probes. No age-related changes in methylation pattern were detected. We can conclude therefore that the methylation pattern of the three X-linked genes is stable during aging in female leukocytes and that a high rate of age-related reactivation of X-linked genes may not be a feature of all X-linked loci. Plenum Publishing Corporation, 1990 Pagani F. Fondazione Rivetti, Laboratorio di Biochimica e Biologia Molecolare, viale Monte Nero 32, 20135, Milan, Italy aut Toniolo D. Istituto di Genetica Biochimica ed Evoluzionistica del CNR, Via Abbiategrasso 207, 27100, Pavia, Italy aut Vergani C. Istituto di Medicina Interna, Universitá degli Studi di Milano, via Pace 9, 20122, Milan, Italy aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/1(1990-01-01), 79-84 0740-7750 16:1<79 1990 16 11188 https://doi.org/10.1007/BF01650482 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01650482 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pagani F. Fondazione Rivetti, Laboratorio di Biochimica e Biologia Molecolare, viale Monte Nero 32, 20135, Milan, Italy aut NATIONALLICENCE 700 1- Toniolo D. Istituto di Genetica Biochimica ed Evoluzionistica del CNR, Via Abbiategrasso 207, 27100, Pavia, Italy aut NATIONALLICENCE 700 1- Vergani C. Istituto di Medicina Interna, Universitá degli Studi di Milano, via Pace 9, 20122, Milan, Italy aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/1(1990-01-01), 79-84 0740-7750 16:1<79 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer