caa a22 4500 465820328 CHVBK 20180323112134.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF01650481 doi (NATIONALLICENCE)springer-10.1007/BF01650481 Gene regulation in reverse transformation: Cyclic AMP-induced actin homolog in CHO cells [Elektronische Daten] [C. Miranti, T. Puck] Reverse transformation (RT) presents a challenge in understanding of the role of protein-genome interaction in regulating gene expression in normal and transformed cells. Early during RT of CHO-K1 cells by cyclic AMP a new protein, mol wt=43,000 and pI=5.3±0.2, was rapidly and specifically induced. This cAMP-induced protein (CIP) is a phosphorylated actin homolog. Induction required new protein synthesis. Actinomycin D treatment failed to inhibit CIP induction, suggesting the existence of an untranslated or sequestered mRNA in untreated cells. Expression of CIP was not dependent upon cell shape or cytoskeletal integrity as are other steps in RT. CIP was detectable only in cAMP-treated cells, whether transformed or nontransformed, and cAMP treatment inhibited growth of both cell types. CIP was associated with soluble cell fractions and not with F-actin. We propose that CIP plays an early role in RT, that is necessary but not sufficient for the complete RT process, and that it participates in the cAMP signaling pathway of cells through changes in the cytoskeleton. This pathway inhibits cell growth as required in the differentiated phenotype. A molecular model is presented for the RT reaction in CHO-K1, which also explains cAMP effects on transformed cells such as the S49 lymphoma and other malignancies. Plenum Publishing Corporation, 1990 Miranti C. Eleanor Roosevelt Institute for Cancer Research, Denver, Colorado aut Puck T. Eleanor Roosevelt Institute for Cancer Research, Denver, Colorado aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/1(1990-01-01), 67-78 0740-7750 16:1<67 1990 16 11188 https://doi.org/10.1007/BF01650481 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01650481 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miranti C. Eleanor Roosevelt Institute for Cancer Research, Denver, Colorado aut NATIONALLICENCE 700 1- Puck T. Eleanor Roosevelt Institute for Cancer Research, Denver, Colorado aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/1(1990-01-01), 67-78 0740-7750 16:1<67 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer