caa a22 4500 465820352 CHVBK 20180323112134.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF01233093 doi (NATIONALLICENCE)springer-10.1007/BF01233093 Zinc treatment, metallothionein expression, and resistance to cisplatin in mouse melanoma cells [Elektronische Daten] [J. Koropatnick, J. Pearson] Metallothioneins (MTs) protect cells from the toxic effects of heavy metals. It has been suggested that they play a role in cellular resistance to alkylating agents and ionizing radiation because of the coincidence of cadmium- and drug-resistance and by virtue of the reactivity of MT with free radicals. We report the analysis of mouse B16 melanoma cell lines with high and low constitutive MT expression. In these cells, both cisplatin and cadmium resistance were associated with constitutive MT accumulation in the absence of heavy-metal induction. However, in cells with high constitutive MT expression (where zinc treatment did not induce increased MT expression), cisplatin resistance, but not cadmium resistance, was increased approximately twofold by zinc treatment. Methotrexate resistance also was increased by zinc treatment in some cases. We conclude that MT is associated with cisplatin resistance, but that effects of heavy-metal treatment other than MT induction are also responsible for cisplatin and methotrexate, but not cadmium, resistance. Plenum Publishing Corporation, 1990 Koropatnick J. The London Regional Cancer Centre, 790 Commissioners Road East, N6A 4L6, London, Ontario, Canada aut Pearson J. The London Regional Cancer Centre, 790 Commissioners Road East, N6A 4L6, London, Ontario, Canada aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/6(1990-11-01), 529-537 0740-7750 16:6<529 1990 16 11188 https://doi.org/10.1007/BF01233093 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01233093 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koropatnick J. The London Regional Cancer Centre, 790 Commissioners Road East, N6A 4L6, London, Ontario, Canada aut NATIONALLICENCE 700 1- Pearson J. The London Regional Cancer Centre, 790 Commissioners Road East, N6A 4L6, London, Ontario, Canada aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/6(1990-11-01), 529-537 0740-7750 16:6<529 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer