caa a22 4500 465820379 CHVBK 20180323112134.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF01233098 doi (NATIONALLICENCE)springer-10.1007/BF01233098 The Chinese hamster V79 cell mutant V-H4 is phenotypically like Fanconi anemia cells [Elektronische Daten] [M. Zdzienicka, F. Arwert, I. Neuteboom, M. Rooimans, J. Simons] It has been shown by genetic complementation analysis that a mitomycin C-sensitive mutant (V-H4) of Chinese hamster V79 cells is the first rodent equivalent of Fanconi anemia (FA) group A. The V-H4 mutant shows many typical characteristics of cells derived from FA patients. V-H4 cells exhibit increased sensitivity towards cross-linking agents as MMC (∼30-fold), cis-DDP (∼10-fold), DEB (∼10-fold), and PUVA (∼1.6-fold), but an only slightly increased sensitivity to monofunctional alkylating agents (EMS and MMS) and actinomycin D. V-H4 cells are also moderately sensitive to adriamycin (1.6-fold), and not sensitive to H2O2. The levels of chromosomal aberrations induced by MMC and cis-DDP treatment are higher (4- to 6-fold) in V-H4 cells than in the wild-type V79 cells. Genetic complementation analysis with other Chinese hamster mutants hypersensitive to MMC (irs1, irs1SF, UV20 and UV41) indicates clearly that V-H4 belongs to a different, new complementation group. This unique mutant is very stable and can serve as a vehicle to isolate the complementing FA-A gene from normal human DNA. Plenum Publishing Corporation, 1990 Zdzienicka M. MGC, Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, Sylvius Laboratory, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands aut Arwert F. Department of Anthropogenetics, Free University, Amsterdam, The Netherlands aut Neuteboom I. MGC, Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, Sylvius Laboratory, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands aut Rooimans M. Department of Anthropogenetics, Free University, Amsterdam, The Netherlands aut Simons J. MGC, Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, Sylvius Laboratory, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/6(1990-11-01), 575-581 0740-7750 16:6<575 1990 16 11188 https://doi.org/10.1007/BF01233098 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01233098 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zdzienicka M. MGC, Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, Sylvius Laboratory, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands aut NATIONALLICENCE 700 1- Arwert F. Department of Anthropogenetics, Free University, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Neuteboom I. MGC, Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, Sylvius Laboratory, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands aut NATIONALLICENCE 700 1- Rooimans M. Department of Anthropogenetics, Free University, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Simons J. MGC, Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, Sylvius Laboratory, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/6(1990-11-01), 575-581 0740-7750 16:6<575 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer