caa a22 4500 465820417 CHVBK 20180323112134.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF01233094 doi (NATIONALLICENCE)springer-10.1007/BF01233094 Complementing mutant alleles define three loci involved in mannosylation of Man5-GlcNAc2-P-P-dolichol in Chinese hamster ovary cells [Elektronische Daten] [Pamela Beck, Mary-Jane Gething, Joseph Sambrook, Mark Lehrman] Dolichol-linked oligosaccharides consisting of twoN-acetylglucosamine, nine mannose, and three glucose residues (Glc3Man9GlcNAc2) are transferred to proteins that contain the consensus sequence Asn-X-Ser/Thr. This transfer occurs upon protein import into the lumen of the endoplasmic reticulum. An intermediate in the biosynthesis of the Glc3Man9GlcNAc2 lipid-linked oligosaccharide contains two GlcNAc and five mannose residues. This intermediate serves as a substrate for further mannosylation and glucosylation before transfer to protein. The addition of the sixth mannose residue to this intermediate requires the enzyme mannosyltransferase VI and the mannose donor, mannose-P-dolichol. Several different CHO cell line mutants that fail to efficiently catalyze this transfer have been described. In this report, we examine seven independent mutant cell lines with various biochemical phenotypes and demonstrate that all can be assigned to one of three genetic complementation groups. One mutation affects mannose-P-dolichol biosynthesis (Lec15), three affect dolichol phosphate biosynthesis (Lec9), and three appear to affect the functional orientation of enzyme substrates (PIR). Plenum Publishing Corporation, 1990 Beck Pamela Department of Biochemistry, University of Texas Southwestern Medical Center, 75235, Dallas, Texas aut Gething Mary-Jane Department of Biochemistry, University of Texas Southwestern Medical Center, 75235, Dallas, Texas aut Sambrook Joseph Department of Biochemistry, University of Texas Southwestern Medical Center, 75235, Dallas, Texas aut Lehrman Mark Department of Pharmacology, University of Texas Southwestern Medical Center, 75235, Dallas, Texas aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/6(1990-11-01), 539-548 0740-7750 16:6<539 1990 16 11188 https://doi.org/10.1007/BF01233094 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01233094 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Beck Pamela Department of Biochemistry, University of Texas Southwestern Medical Center, 75235, Dallas, Texas aut NATIONALLICENCE 700 1- Gething Mary-Jane Department of Biochemistry, University of Texas Southwestern Medical Center, 75235, Dallas, Texas aut NATIONALLICENCE 700 1- Sambrook Joseph Department of Biochemistry, University of Texas Southwestern Medical Center, 75235, Dallas, Texas aut NATIONALLICENCE 700 1- Lehrman Mark Department of Pharmacology, University of Texas Southwestern Medical Center, 75235, Dallas, Texas aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/6(1990-11-01), 539-548 0740-7750 16:6<539 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer