caa a22 4500 465820425 CHVBK 20180323112134.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF01233091 doi (NATIONALLICENCE)springer-10.1007/BF01233091 Molecular cloning of a mammalian gene involved in the fixation of UV-induced mutations [Elektronische Daten] [S. Bouffler, D. Godfrey, M. Raman, S. Musk, R. Johnson] A mammalian DNA damage tolerance gene has been isolated by DNA transfection and cosmid rescue. Following cotransfection of mouse genomic DNA and pSV2neo into SVM, the UV hypersensitive mutant Indian muntjac cell line, clones with a 1.7 to 2.0-fold greater D37 value for UV killing were isolated. This trait was carried through three rounds of transfection. A neo gene and flanking sequences from a tertiary transfectant were cloned by cosmid rescue. The cosmid clone confers UV resistance to SVM and improves the ability of the cell to replicate UV damaged DNA. This replication appears to be error-prone; UV-induced 6-thioguanine-resistant mutants occur four to fivefold more frequently than in SVM or a wild-type Indian muntjac line. Thus, the gene isolated is not homologous to that defective in SVM. We believe that this is the first mammalian gene to be isolated that is directly involved in mutation fixation. Plenum Publishing Corporation, 1990 Bouffler S. CRC Mammalian Cell DNA Repair Research Group, Department of Zoology, University of Cambridge, CB2 3EJ, Cambridge, UK aut Godfrey D. CRC Mammalian Cell DNA Repair Research Group, Department of Zoology, University of Cambridge, CB2 3EJ, Cambridge, UK aut Raman M. CRC Mammalian Cell DNA Repair Research Group, Department of Zoology, University of Cambridge, CB2 3EJ, Cambridge, UK aut Musk S. CRC Mammalian Cell DNA Repair Research Group, Department of Zoology, University of Cambridge, CB2 3EJ, Cambridge, UK aut Johnson R. CRC Mammalian Cell DNA Repair Research Group, Department of Zoology, University of Cambridge, CB2 3EJ, Cambridge, UK aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/6(1990-11-01), 507-516 0740-7750 16:6<507 1990 16 11188 https://doi.org/10.1007/BF01233091 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01233091 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bouffler S. CRC Mammalian Cell DNA Repair Research Group, Department of Zoology, University of Cambridge, CB2 3EJ, Cambridge, UK aut NATIONALLICENCE 700 1- Godfrey D. CRC Mammalian Cell DNA Repair Research Group, Department of Zoology, University of Cambridge, CB2 3EJ, Cambridge, UK aut NATIONALLICENCE 700 1- Raman M. CRC Mammalian Cell DNA Repair Research Group, Department of Zoology, University of Cambridge, CB2 3EJ, Cambridge, UK aut NATIONALLICENCE 700 1- Musk S. CRC Mammalian Cell DNA Repair Research Group, Department of Zoology, University of Cambridge, CB2 3EJ, Cambridge, UK aut NATIONALLICENCE 700 1- Johnson R. CRC Mammalian Cell DNA Repair Research Group, Department of Zoology, University of Cambridge, CB2 3EJ, Cambridge, UK aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/6(1990-11-01), 507-516 0740-7750 16:6<507 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer