caa a22 4500 465820433 CHVBK 20180323112134.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF01233099 doi (NATIONALLICENCE)springer-10.1007/BF01233099 Bromodeoxyuridine- and cyclic AMP-mediated regulation of tyrosinase in Syrian hamster melanoma cells [Elektronische Daten] [Sikha Rauth, George Hoganson, Richard Davidson] The thymidine analog 5-bromodeoxyuridine (BrdU) suppresses pigmentation and tyrosinase activity in Syrian hamster melanoma cells W1-1-1. Studies on the molecular mechanism of suppression of pigmentation indicated that BrdU treatment affects the level of tyrosinase gene transcripts. No detectable tyrosinase message was found by Northern blot analysis in cells cultured in the presence of BrdU at concentrations even as low as 0.2 µM. The level of tyrosinase mRNA was found to reflect the level of pigmentation and tyrosinase activity. Studies with dibutyryl cyclic AMP (cAMP) showed that it inhibited pigment synthesis in W1-1-1 cells. With increasing concentrations of cAMP ranging from 10 µM to 300 µM, pigmentation and tyrosinase activity decreased progressively. This inhibition was found to be associated with a corresponding decrease in the level of tyrosinase mRNA. W1-1-1 cells were found not to respond to melanocyte stimulating hormone (MSH). There was no change in pigmentation, tyrosinase activity, or tyrosinase mRNA level in W1-1-1 cells in the presence of MSH. Similarly, theophylline, a phosphodiesterase inhibitor, had no effect on pigmentation or tyrosinase activity in W1-1-1 cells. Plenum Publishing Corporation, 1990 Rauth Sikha Department of Genetics, University of Illinois College of Medicine, 808 South Wood Street, 60612, Chicago, Illinois aut Hoganson George Department of Pediatrics, University of Illinois College of Medicine, 808 South Wood Street, 60612, Chicago, Illinois aut Davidson Richard Department of Genetics, University of Illinois College of Medicine, 808 South Wood Street, 60612, Chicago, Illinois aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/6(1990-11-01), 583-592 0740-7750 16:6<583 1990 16 11188 https://doi.org/10.1007/BF01233099 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01233099 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rauth Sikha Department of Genetics, University of Illinois College of Medicine, 808 South Wood Street, 60612, Chicago, Illinois aut NATIONALLICENCE 700 1- Hoganson George Department of Pediatrics, University of Illinois College of Medicine, 808 South Wood Street, 60612, Chicago, Illinois aut NATIONALLICENCE 700 1- Davidson Richard Department of Genetics, University of Illinois College of Medicine, 808 South Wood Street, 60612, Chicago, Illinois aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/6(1990-11-01), 583-592 0740-7750 16:6<583 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer