caa a22 4500 465820476 CHVBK 20180323112134.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF01233360 doi (NATIONALLICENCE)springer-10.1007/BF01233360 Physical mapping of chromosome 21 DNA markers in Alzheimer's disease region using somatic cell hybrids [Elektronische Daten] [Guy Van Camp, Wim Van Hul, Hubert Backhovens, Piet Stinissen, Anita Wehnert, David Patterson, Antoon Vandenberghe, Christine Van Broeckhoven] From a chromosome 21 phage library, we selected 10 clones located proximal of the senile plaque amyloid precursor protein gene. Since a locus for Alzheimer's disease (AD) has been localized in the pericentromeric region of chromosome 21, the selected phage clones are potential candidate probes for genetic analysis of AD. In this study, we subcloned single-copy fragments of the selected phage clones, refined their physical localization, and examined their chromosomal distribution in relation to their position on chromosome 21. The results indicated that the phage clones are identifying nine chromosome 21 loci, which, if polymorphic, may be helpful in localizing the AD locus more precisely. Moreover, since all phage clones are located close to the centromere of chromosome 21, they can be used to determine the parental origin of nondisjunction in trisomy 21 with high reliability. Plenum Publishing Corporation, 1990 Van Camp Guy Department of Biochemistry, University of Antwerp (UIA), Universiteitsplein 1, B-2610, Antwerpen, Belgium aut Van Hul Wim Department of Biochemistry, University of Antwerp (UIA), Universiteitsplein 1, B-2610, Antwerpen, Belgium aut Backhovens Hubert Department of Biochemistry, University of Antwerp (UIA), Universiteitsplein 1, B-2610, Antwerpen, Belgium aut Stinissen Piet Department of Biochemistry, University of Antwerp (UIA), Universiteitsplein 1, B-2610, Antwerpen, Belgium aut Wehnert Anita Department of Biochemistry, University of Antwerp (UIA), Universiteitsplein 1, B-2610, Antwerpen, Belgium aut Patterson David Eleanor Roosevelt Institute, 1899 Gaylord Street, 80206, Denver, Colorado aut Vandenberghe Antoon Department of Biochemistry, University of Antwerp (UIA), Universiteitsplein 1, B-2610, Antwerpen, Belgium aut Van Broeckhoven Christine Department of Biochemistry, University of Antwerp (UIA), Universiteitsplein 1, B-2610, Antwerpen, Belgium aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/3(1990-05-01), 241-249 0740-7750 16:3<241 1990 16 11188 https://doi.org/10.1007/BF01233360 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01233360 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Van Camp Guy Department of Biochemistry, University of Antwerp (UIA), Universiteitsplein 1, B-2610, Antwerpen, Belgium aut NATIONALLICENCE 700 1- Van Hul Wim Department of Biochemistry, University of Antwerp (UIA), Universiteitsplein 1, B-2610, Antwerpen, Belgium aut NATIONALLICENCE 700 1- Backhovens Hubert Department of Biochemistry, University of Antwerp (UIA), Universiteitsplein 1, B-2610, Antwerpen, Belgium aut NATIONALLICENCE 700 1- Stinissen Piet Department of Biochemistry, University of Antwerp (UIA), Universiteitsplein 1, B-2610, Antwerpen, Belgium aut NATIONALLICENCE 700 1- Wehnert Anita Department of Biochemistry, University of Antwerp (UIA), Universiteitsplein 1, B-2610, Antwerpen, Belgium aut NATIONALLICENCE 700 1- Patterson David Eleanor Roosevelt Institute, 1899 Gaylord Street, 80206, Denver, Colorado aut NATIONALLICENCE 700 1- Vandenberghe Antoon Department of Biochemistry, University of Antwerp (UIA), Universiteitsplein 1, B-2610, Antwerpen, Belgium aut NATIONALLICENCE 700 1- Van Broeckhoven Christine Department of Biochemistry, University of Antwerp (UIA), Universiteitsplein 1, B-2610, Antwerpen, Belgium aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/3(1990-05-01), 241-249 0740-7750 16:3<241 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer