caa a22 4500 465820484 CHVBK 20180323112134.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF01233359 doi (NATIONALLICENCE)springer-10.1007/BF01233359 Sublocalization on chromosome 21 of human interferon-alpha receptor gene and the gene for an interferon-gamma response protein [Elektronische Daten] [Jerome Langer, Abbas Rashidbaigi, Li-Wen Lai, David Patterson, Carol Jones] The cellular responses to alpha and beta interferons (IFN-α and -β) are mediated through the IFN-α/β (type I) receptor, while the response to IFN-γ is mediated through the IFN-γ (type II) receptor. The receptors for IFN-α/β and IFN-γ are encoded by genes on human chromosomes 21 and 6q, respectively. The presence of chromosome 21q confers both ligand binding and responsiveness to human IFN-α/β, whereas chromosome 6q confers binding of Hu-IFN-γ, but not cellular responsiveness on somatic cell hybrids. Chromosome 6q (i.e., the Hu-IFN-γ receptor gene) and chromosome 21q are both necessary for the cellular response of somatic cell hybrids (from fibroblasts) to Hu-IFN-γ. It is conceivable that the factor mediating activity through the IFN-γ receptor is, in fact, the IFN-α receptor, or that the two genes are distinct but part of an "interferon response” region. Here we more precisely localize on human chromosome 21 the genes for the IFN-α receptor and for the factor(s) mediating the action of IFN-γ through the chromosome 6-encoded receptor. Hamster-human somatic cell hybrids containing various fragments of human chromosome 21 were used. The presence of the human IFN-α/β receptor was determined by binding32P-labeled human IFN-α to cells, covalently cross-linking the [32P]IFN-α-receptor complex, and analyzing it by SDS-polyacrylamide gel electrophoresis. The presence of the IFN-γ receptor-related factor mediating cellular responsiveness was determined by HLA induction in hybrid cells containing the IFN-γ receptor (chromosome 6q), a transfected copy of the human HLA-B7 gene, and various portions of chromosome 21. In all hybrids examined, the two genes cosegregate. Specifically, both genes are localized to the region of chromosome 21 containing the markers D21S58, D21S65, and GART and appear to be proximal to D21S58. The implications for IFN action are discussed. Plenum Publishing Corporation, 1990 Langer Jerome Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 675 Hoes Lane, 08854-5635, Piscataway, New Jersey aut Rashidbaigi Abbas Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 675 Hoes Lane, 08854-5635, Piscataway, New Jersey aut Lai Li-Wen Eleanor Roosevelt Institute for Cancer Research, 1899 Gaylord Street, 80206, Denver, Colorado aut Patterson David Eleanor Roosevelt Institute for Cancer Research, 1899 Gaylord Street, 80206, Denver, Colorado aut Jones Carol Eleanor Roosevelt Institute for Cancer Research, 1899 Gaylord Street, 80206, Denver, Colorado aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/3(1990-05-01), 231-240 0740-7750 16:3<231 1990 16 11188 https://doi.org/10.1007/BF01233359 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01233359 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Langer Jerome Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 675 Hoes Lane, 08854-5635, Piscataway, New Jersey aut NATIONALLICENCE 700 1- Rashidbaigi Abbas Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 675 Hoes Lane, 08854-5635, Piscataway, New Jersey aut NATIONALLICENCE 700 1- Lai Li-Wen Eleanor Roosevelt Institute for Cancer Research, 1899 Gaylord Street, 80206, Denver, Colorado aut NATIONALLICENCE 700 1- Patterson David Eleanor Roosevelt Institute for Cancer Research, 1899 Gaylord Street, 80206, Denver, Colorado aut NATIONALLICENCE 700 1- Jones Carol Eleanor Roosevelt Institute for Cancer Research, 1899 Gaylord Street, 80206, Denver, Colorado aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/3(1990-05-01), 231-240 0740-7750 16:3<231 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer