caa a22 4500 465820506 CHVBK 20180323112134.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF01233358 doi (NATIONALLICENCE)springer-10.1007/BF01233358 Loss of alleles in aprt mutants of CHO cells demonstrated by BclI restriction-fragment-length variation [Elektronische Daten] [Pascale Dewyse, W. Bradley] A BclI restriction-fragment-length variation (RFLV) has been identified 7 kb downstream of the 3′ end of the adenine phosphoribosyltransferase (aprt) gene in the Chinese hamster CHO cell line. This RFLV generates 19 kb and 17 kb+2 kb BclI fragments. Three other cell lines derived from different Chinese hamsters are homozygous for this RFLV. The 17 kb+2 kb allele was mapped to the Z4 chromosome based upon the absence of this allele in the CHO-derived hemizygote AA8-5, whose functional allele is known to map to chromosome Z7. The class 2aprt +/-line D416 loses the 19-kb allele after undergoing the high-frequency (HF) deletion event, indicating that the HF deletion event occurs on the Z7 chromosome. On the other hand, deletion events in the class 3 heterozygote D423 (which do not spontaneously occur at HF) resulted in loss of the 17 kb+2 kb allele. This is consistent with a previously proposed model holding that the twoaprt alleles in the CHO cell line have different inherent probabilities of undergoing a large deletion event. Plenum Publishing Corporation, 1990 Dewyse Pascale Institut du Cancer de Montréal, 1560 est, Sherbrooke, H2L 4M1, Montréal, Québec, Canada aut Bradley W. Institut du Cancer de Montréal, 1560 est, Sherbrooke, H2L 4M1, Montréal, Québec, Canada aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/3(1990-05-01), 225-230 0740-7750 16:3<225 1990 16 11188 https://doi.org/10.1007/BF01233358 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01233358 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dewyse Pascale Institut du Cancer de Montréal, 1560 est, Sherbrooke, H2L 4M1, Montréal, Québec, Canada aut NATIONALLICENCE 700 1- Bradley W. Institut du Cancer de Montréal, 1560 est, Sherbrooke, H2L 4M1, Montréal, Québec, Canada aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/3(1990-05-01), 225-230 0740-7750 16:3<225 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer