caa a22 4500 465820522 CHVBK 20180323112134.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF01233357 doi (NATIONALLICENCE)springer-10.1007/BF01233357 Lectin-resistant CHO cells: Selection of seven new mutants resistant to ricin [Elektronische Daten] [Pamela Stanley, Sandra Sallustio, Sharon Krag, Barbara Dunn] In attempts to isolate new CHO glycosylation mutants, selection protocols using plant lectins that bind galactose residues of cell surface carbohydrates were applied to mutagenized CHO populations. The lectins were used alone or in combination to obtain seven ricin-resistant phenotypes. Each mutant had distinctive properties compared with previously described ricin-resistant CHO cells. One of the new phenotypes was dominant in somatic cell hybrids, and the others were recessive. Complementation analyses between related lectinresistant (LecR) phenotypes indicated that each new isolate represented a novel genotype. Five of the mutants had properties typical of new CHO glycosylation mutants. The remaining two mutants were not readily categorized. Although they did not appear to be ricin-internalization or protein-synthesis mutants, they also did not display the marked alterations in sensitivity to several lectins of different sugar specificity expected for glycosylation mutants. The seven new LecR mutants described in these studies brings the total number of different LecR CHO mutants isolated by this and other laboratories to about 40. Criteria for identifying new LecR mutations in CHO cells are discussed. Plenum Publishing Corporation, 1990 Stanley Pamela Department Cell Biology, Albert Einstein College of Medicine, 10461, Bronx, New York aut Sallustio Sandra Department Cell Biology, Albert Einstein College of Medicine, 10461, Bronx, New York aut Krag Sharon Department Biochemistry, Johns Hopkins School Hygiene and Public Health, 21205, Baltimore, Maryland aut Dunn Barbara Department Cell Biology, Albert Einstein College of Medicine, 10461, Bronx, New York aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/3(1990-05-01), 211-223 0740-7750 16:3<211 1990 16 11188 https://doi.org/10.1007/BF01233357 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01233357 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stanley Pamela Department Cell Biology, Albert Einstein College of Medicine, 10461, Bronx, New York aut NATIONALLICENCE 700 1- Sallustio Sandra Department Cell Biology, Albert Einstein College of Medicine, 10461, Bronx, New York aut NATIONALLICENCE 700 1- Krag Sharon Department Biochemistry, Johns Hopkins School Hygiene and Public Health, 21205, Baltimore, Maryland aut NATIONALLICENCE 700 1- Dunn Barbara Department Cell Biology, Albert Einstein College of Medicine, 10461, Bronx, New York aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/3(1990-05-01), 211-223 0740-7750 16:3<211 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer