caa a22 4500 465820573 CHVBK 20180323112134.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF01232460 doi (NATIONALLICENCE)springer-10.1007/BF01232460 Relative frequencies of homologous recombination between plasmids introduced into DNA repair-deficient and other mammalian somatic cell lines [Elektronische Daten] [Wayne Wahls, Peter Moore] Twelve mammalian somatic cell lines, some of them DNA damage-sensitive mutants paired with their respective wild-type parental lines, were assayed for their ability to catalyze extrachromosomal, intermolecular homologous recombination between pSV2neo plasmid recombination substrates. All of the somatic cell lines analyzed are capable of catalyzing homologous recombination; however, there is a wide range of efficiencies with which they do so. Five human cell lines display a fourfold range of recombination frequencies, and six hamster cell lines vary almost 20-fold. Linearizing one of the recombination substrates stimulates recombination in all but one of the cell lines. Two of the three paired mutant cell lines display a threefold reduction in their ability to catalyze homologous recombination when compared to their respective parental cell lines, indicating that the mutations that render them sensitive to DNA damaging agents might also play a role in homologous recombination. Plenum Publishing Corporation, 1990 Wahls Wayne Department of Genetics, The University of Illinois at Chicago, 1404 CME, M/C 669, 808 South Wood Street, 60612, Chicago, Illinois aut Moore Peter Department of Genetics, The University of Illinois at Chicago, 1404 CME, M/C 669, 808 South Wood Street, 60612, Chicago, Illinois aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/4(1990-07-01), 321-329 0740-7750 16:4<321 1990 16 11188 https://doi.org/10.1007/BF01232460 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01232460 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wahls Wayne Department of Genetics, The University of Illinois at Chicago, 1404 CME, M/C 669, 808 South Wood Street, 60612, Chicago, Illinois aut NATIONALLICENCE 700 1- Moore Peter Department of Genetics, The University of Illinois at Chicago, 1404 CME, M/C 669, 808 South Wood Street, 60612, Chicago, Illinois aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/4(1990-07-01), 321-329 0740-7750 16:4<321 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer