caa a22 4500 465820581 CHVBK 20180323112134.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF01232457 doi (NATIONALLICENCE)springer-10.1007/BF01232457 Selective enrichment for temperature-sensitive secretion mutants of mammalian cells using plant lectin, concanavalin A [Elektronische Daten] [Kenneth Henry II, Jerry Brown, Ardythe McCracken] The use of conditional mutants as a genetic approach to study protein secretion in mammalian cells requires the isolation of a large number of mutants. Because a procedure for the direct selection of mutants with secretion defencts is not available, their isolation depends upon the selective enrichment of mutant phenotypes in a cell population. We have devised an enrichment strategy in which rat hepatoma cells unable to replace surface membrane receptors of a plant lectin, concanavalin A, are resistant to the cytotoxic effects of this lectin when administered at a nonpermissive temperature. This treatment yields a population highly enriched in cells that demonstrate temperature-sensitive secretion. Therefore, this selection strategy has important application in isolating temperature-sensitive mutants for use in the study of the mammalian cell secretion pathway. Plenum Publishing Corporation, 1990 Henry II Kenneth Department of Biology, University of Nevada, 89557, Reno, Nevada aut Brown Jerry Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, 80262, Denver, Colorado aut McCracken Ardythe Department of Biology, University of Nevada, 89557, Reno, Nevada aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/4(1990-07-01), 297-304 0740-7750 16:4<297 1990 16 11188 https://doi.org/10.1007/BF01232457 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01232457 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Henry II Kenneth Department of Biology, University of Nevada, 89557, Reno, Nevada aut NATIONALLICENCE 700 1- Brown Jerry Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, 80262, Denver, Colorado aut NATIONALLICENCE 700 1- McCracken Ardythe Department of Biology, University of Nevada, 89557, Reno, Nevada aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/4(1990-07-01), 297-304 0740-7750 16:4<297 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer