caa a22 4500 465820611 CHVBK 20180323112134.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF01232465 doi (NATIONALLICENCE)springer-10.1007/BF01232465 Antisense RNA inhibition of HPRT synthesis [Elektronische Daten] [J. Stout, C. Caskey] The abundant production of antisense hypoxanthine phosphoribosyltransferase (HPRT) RNA in NIH-3T3, COS, or HeLa cells leads to an inhibition of HPRT synthesis. HPRT enzyme levels in cells transfected with mouse HPRT antisense RNA expression vectors are reduced to less than 1% of parental cell activity, resulting in resistance to 6-thioguanine (6TG). The expression of antisense HPRT RNA leads to a marked reduction in the steady-state levels of endogenous HPRT mRNA. Furthermore, we demonstrate that intron-specific antisense RNA, complementary to sequences adjacent to splice donor or acceptor sites of the first intron of the mouse HPRT gene, are effective in depressing endogenous HPRT levels. These studies suggest that antisense RNA can inhibit gene expression in the nucleus, possibly by perturbing nuclear RNA processing. Plenum Publishing Corporation, 1990 Stout J. Institute for Molecular Genetics, Baylor College of Medicine, 77030, Houston, Texas aut Caskey C. Institute for Molecular Genetics, Baylor College of Medicine, 77030, Houston, Texas aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/4(1990-07-01), 369-382 0740-7750 16:4<369 1990 16 11188 https://doi.org/10.1007/BF01232465 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01232465 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stout J. Institute for Molecular Genetics, Baylor College of Medicine, 77030, Houston, Texas aut NATIONALLICENCE 700 1- Caskey C. Institute for Molecular Genetics, Baylor College of Medicine, 77030, Houston, Texas aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/4(1990-07-01), 369-382 0740-7750 16:4<369 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer