caa a22 4500 465820662 CHVBK 20180323112135.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF01232459 doi (NATIONALLICENCE)springer-10.1007/BF01232459 Deregulation of DNA polymerase β by sense and antisense RNA expression in mouse 3T3 cells alters cell growth [Elektronische Daten] [Barbara Zmudzka, Samuel Wilson] DNA polymerase beta (β-pol) and its mRNA are maintained at constitutive levels during the cell cycle and during stages of cell growth in culture. To study biological consequences of variations in the level of this DNA repair enzyme and/or its mRNA, we prepared expression vectors in which cDNA for human β-pol is inserted under the control of a metallothionein promoter (pMT) in the sense and antisense orientation, respectively, and these vectors then were used for stable transformation of mouse 3T3 cells. Vectors also contained the mouseDHFR gene, such that culture of transformants in medium with increasing concentrations of methotrexate resulted in amplification of inserted DNA. The levels of sense and antisense transcripts are strongly increased by culture of transformants in medium with 65 µM Zn2+, although some expression is detected even without Zn2+ induction. After five days of induction, the β-pol level was about threefold higher in sense cells and about 10-fold lower in antisense cells than in parallel cultures without induction. The antisense line has a threefold increased cell doubling time in the presence of 65 µM Zn2+ compared with the absence of Zn2+. Zn2+ (65 µM) induction for the sense line results in normal growth for the first three days and, thereafter, a complete cessation of growth. Yet, these blocked cells remain fully viable. The results indicate that sudden deregulation of β-pol expression alters cell growth in mouse 3T3 cells. Plenum Publishing Corporation, 1990 Zmudzka Barbara Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, 20892, Bethesda, Maryland aut Wilson Samuel Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, 20892, Bethesda, Maryland aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/4(1990-07-01), 311-320 0740-7750 16:4<311 1990 16 11188 https://doi.org/10.1007/BF01232459 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01232459 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zmudzka Barbara Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, 20892, Bethesda, Maryland aut NATIONALLICENCE 700 1- Wilson Samuel Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, 20892, Bethesda, Maryland aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/4(1990-07-01), 311-320 0740-7750 16:4<311 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer