caa a22 4500 465820697 CHVBK 20180323112135.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF01232466 doi (NATIONALLICENCE)springer-10.1007/BF01232466 Antisense RNA production in transgenic mice [Elektronische Daten] [M. Munir, Belinda Rossiter, C. Caskey] There are many reports of antisense inhibition of gene expression in cultured cells. We have generated four strains of transgenic mice expressing antisense hypoxanthine guanine phosphoribosyltransferase (HPRT) RNA in brain, or heart and liver, or all three organs. In the brain of one strain, the level of antisense RNA in the different brain regions roughly correlates with the degree of inhibition of the native HPRT mRNA in those same regions. Despite this decrease of up to 60% of endogenous HPRT mRNA, no reproducible reduction in HPRT activity has been observed. Possible reasons for the differences between the effectiveness of antisense inhibition in cultured cells and transgenic animals are discussed. Plenum Publishing Corporation, 1990 Munir M. Howard Hughes Medical Institute, Baylor College of Medicine, 77030, Houston, Texas aut Rossiter Belinda Institute for Molecular Genetics, Baylor College of Medicine, 77030, Houston, Texas aut Caskey C. Howard Hughes Medical Institute, Baylor College of Medicine, 77030, Houston, Texas aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/4(1990-07-01), 383-394 0740-7750 16:4<383 1990 16 11188 https://doi.org/10.1007/BF01232466 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01232466 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Munir M. Howard Hughes Medical Institute, Baylor College of Medicine, 77030, Houston, Texas aut NATIONALLICENCE 700 1- Rossiter Belinda Institute for Molecular Genetics, Baylor College of Medicine, 77030, Houston, Texas aut NATIONALLICENCE 700 1- Caskey C. Howard Hughes Medical Institute, Baylor College of Medicine, 77030, Houston, Texas aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/4(1990-07-01), 383-394 0740-7750 16:4<383 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer