caa a22 4500 465820727 CHVBK 20180323112135.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF01233195 doi (NATIONALLICENCE)springer-10.1007/BF01233195 Multiple pathways of DNA double-strand break processing in a mutant Indian muntjac cell line [Elektronische Daten] [S. Bouffler, B. Jha, R. Johnson] DNA break processing is compared in the Indian muntjac cell lines, SVM and DM. The initial frequencies and resealing of X-ray generated single- and double-strand breaks are similar in the two cell lines. Inhibiting the repair of UV damage leads to greater double-strand breakage in SVM than in DM, and some of these breaks are not repaired; however, repair-associated single-strand breakage and resealing are normal. Dimethylsulfate also induces excess double-strand breakage in SVM, and these breaks are irreparable. Restricted plasmids are reconstituted correctly in SVM at approximately 30% of the frequency observed in DM. Thus SVM has a reduced capacity to repair certain types of double-strand break. This defect is not due to a DNA ligase deficiency. We conclude that DNA double-strand breaks are repaired by a variety of pathways within mammalian cells and that the structure of the break or its mode of formation determines its subsequent fate. Plenum Publishing Corporation, 1990 Bouffler S. CRC Mammalian Cell DNA Repair Research Group, Department of Zoology, University of Cambridge, CB2 3EJ, 0223 336627, Cambridge, U.K. aut Jha B. CRC Mammalian Cell DNA Repair Research Group, Department of Zoology, University of Cambridge, CB2 3EJ, 0223 336627, Cambridge, U.K. aut Johnson R. CRC Mammalian Cell DNA Repair Research Group, Department of Zoology, University of Cambridge, CB2 3EJ, 0223 336627, Cambridge, U.K. aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/5(1990-09-01), 451-460 0740-7750 16:5<451 1990 16 11188 https://doi.org/10.1007/BF01233195 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01233195 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bouffler S. CRC Mammalian Cell DNA Repair Research Group, Department of Zoology, University of Cambridge, CB2 3EJ, 0223 336627, Cambridge, U.K aut NATIONALLICENCE 700 1- Jha B. CRC Mammalian Cell DNA Repair Research Group, Department of Zoology, University of Cambridge, CB2 3EJ, 0223 336627, Cambridge, U.K aut NATIONALLICENCE 700 1- Johnson R. CRC Mammalian Cell DNA Repair Research Group, Department of Zoology, University of Cambridge, CB2 3EJ, 0223 336627, Cambridge, U.K aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/5(1990-09-01), 451-460 0740-7750 16:5<451 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer