caa a22 4500 465820751 CHVBK 20180323112135.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF01233196 doi (NATIONALLICENCE)springer-10.1007/BF01233196 Isolation of temperature-sensitive CHO-K1 cell mutants exhibiting chromosomal instability and reduced DNA synthesis at nonpermissive temperature [Elektronische Daten] [Hideo Tsuji, Yasushi Matsudo, Satsuki Tsuji, Fumio Hanaoka, Masao Hyodo, Tada-aki Hori] Twenty-five temperature-sensitive (ts) mutants were isolated from Chinese hamster CHO-K1 cells after mutagenization withN-methyl-N′-nitro-N-nitrosoguanidine. Of 13 complementation groups identified, nine exhibited chromosomal instability at a nonpermissive temperature. They were classified into three major classes according to inducibility of sister chromatid exchange (SCE) and/or chromosomal aberration (CA): class 1 resulted in predominant SCEs, class 2 manifested both SCEs and CAs, and class 3 exhibited higher induction of CAs. Flow cytometric analysis of the mutants exhibiting chromosomal instability indicated that many of the mutants were arrested in the S or S to G2 phases of the cell cycle at the nonpermissive temperature, accompanied by a decrease in the rate of DNA synthesis. These results imply that ts defects are related to some points in DNA replication and might be responsible for the induction of SCEs and/or CAs at the nonpermissive temperature. Plenum Publishing Corporation, 1990 Tsuji Hideo Division of Genetics, National Institute of Radiological Sciences, 9-1, Anagawa-4, 260, Chiba, Japan aut Matsudo Yasushi Division of Genetics, National Institute of Radiological Sciences, 9-1, Anagawa-4, 260, Chiba, Japan aut Tsuji Satsuki Division of Genetics, National Institute of Radiological Sciences, 9-1, Anagawa-4, 260, Chiba, Japan aut Hanaoka Fumio Radiation Biology Laboratory, Institute of Physical and Chemical Research, 2-1 Hirosawa, Wako, 351-01, Saitama, Japan aut Hyodo Masao Department of Molecular Biology, Tokai University School of Medicine, Boseidai, Isehara, 259-11, Kanagawa, Japan aut Hori Tada-aki Division of Genetics, National Institute of Radiological Sciences, 9-1, Anagawa-4, 260, Chiba, Japan aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/5(1990-09-01), 461-476 0740-7750 16:5<461 1990 16 11188 https://doi.org/10.1007/BF01233196 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01233196 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tsuji Hideo Division of Genetics, National Institute of Radiological Sciences, 9-1, Anagawa-4, 260, Chiba, Japan aut NATIONALLICENCE 700 1- Matsudo Yasushi Division of Genetics, National Institute of Radiological Sciences, 9-1, Anagawa-4, 260, Chiba, Japan aut NATIONALLICENCE 700 1- Tsuji Satsuki Division of Genetics, National Institute of Radiological Sciences, 9-1, Anagawa-4, 260, Chiba, Japan aut NATIONALLICENCE 700 1- Hanaoka Fumio Radiation Biology Laboratory, Institute of Physical and Chemical Research, 2-1 Hirosawa, Wako, 351-01, Saitama, Japan aut NATIONALLICENCE 700 1- Hyodo Masao Department of Molecular Biology, Tokai University School of Medicine, Boseidai, Isehara, 259-11, Kanagawa, Japan aut NATIONALLICENCE 700 1- Hori Tada-aki Division of Genetics, National Institute of Radiological Sciences, 9-1, Anagawa-4, 260, Chiba, Japan aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/5(1990-09-01), 461-476 0740-7750 16:5<461 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer