caa a22 4500 465820794 CHVBK 20180323112135.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF01233192 doi (NATIONALLICENCE)springer-10.1007/BF01233192 Monochromosomal mouse microcell hybrids containing inserted selectable neo genes [Elektronische Daten] [T. Sidén, M. Höglund, D. Röhme] Normal mouse fibroblasts at early passage levels were used as a starting material to construct mouse-hamster microcell hybrids (MCH). Theneo r gene, carried on the pSV2neo and pZIP-NeoSV(X)1 plasmids, was introduced into the mouse fibroblasts by gene transfection and retroviral infection, respectively, prior to microcell hybridization into the E36 Chinese hamster cell line. In total about 180 MCH clones were isolated and their amount of mouse DNA was estimated by dot-blot analysis. About 50% of the transfection based hybrids (T-hybrids) showed signals indicating one mouse chromosome, less than 10% more than one mouse chromosome, and the remaining clones contained only subchromosomal amounts of mouse DNA. In the infection-based hybrid series (I-hybrids) more than 95% showed only subchromosomal mouse DNA content. Chromosomal integration analysis verified the presence ofneo r insertions in all 42 hybrid clones analyzed. C-banding analysis verified 14 of 15 hybrids scored as monochromosomals on dot blots. Chromosome fragmentation in T-type MCH was found to be (1) nonrandom, preferentially occurring in MCH derived from certain transfectants, (2) late in clonal establishment, and (3) essentially not related to prolonged cultivation in vitro. Once established, most T-type MCH clones including mono- and subchromosomal hybrids were essentially stable during prolonged cultivation. In contrast MCH initially containing several mouse chromosomes tend to lose the nonselectable ones during prolonged cultivation. In total we estimate the number of independent monochromosomal MCH derived in this study to more than 30. Plenum Publishing Corporation, 1990 Sidén T. Department of Molecular Genetics, Institute of Genetics, University of Lund, Sölvegatan 29, S-223 62, Lund, Sweden aut Höglund M. Department of Molecular Genetics, Institute of Genetics, University of Lund, Sölvegatan 29, S-223 62, Lund, Sweden aut Röhme D. Department of Molecular Genetics, Institute of Genetics, University of Lund, Sölvegatan 29, S-223 62, Lund, Sweden aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/5(1990-09-01), 425-435 0740-7750 16:5<425 1990 16 11188 https://doi.org/10.1007/BF01233192 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01233192 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sidén T. Department of Molecular Genetics, Institute of Genetics, University of Lund, Sölvegatan 29, S-223 62, Lund, Sweden aut NATIONALLICENCE 700 1- Höglund M. Department of Molecular Genetics, Institute of Genetics, University of Lund, Sölvegatan 29, S-223 62, Lund, Sweden aut NATIONALLICENCE 700 1- Röhme D. Department of Molecular Genetics, Institute of Genetics, University of Lund, Sölvegatan 29, S-223 62, Lund, Sweden aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/5(1990-09-01), 425-435 0740-7750 16:5<425 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer