caa a22 4500 465820816 CHVBK 20180323112135.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF01233045 doi (NATIONALLICENCE)springer-10.1007/BF01233045 A dominant negative mutation in two proteins created by ectopic expression of an AU-rich 3′ untranslated region [Elektronische Daten] [Bruce Bunnell, Helen Fillmore, Paula Gregory, Vincent Kidd] We have found that the ectopic expression of a specific gene's 3′ untranslated region leads to the dose dependent loss, relative to gene copy number, of that specific mRNA and protein, as well as an associated protein, in a eukaryotic cell line. The loss of these proteins from the eukaryotic cell line also results in specific phenotypic changes in these cells, suggesting that we have created a dominant negative mutant. This gene's 3′ untranslated region is known to contain numerous AU sequences, reminiscent of other eukaryotic genes whose expression may be regulated by these sequences. The apparent control of gene expression by a truncated 3′ untranslated region sequence provides further evidence supporting the regulatory function of these regions. The resulting decrease in steady-state mRNA levels by the overexpression of a portion of that gene's 3′ untranslated region further suggests the possible existence of a factor(s) that may bind to this region, and thus regulate gene function via its mRNA. The use of a gene's 3′ untranslated sequence to create a specific dominant negative mutation may also be applicable to other eukaryotic genes whose expression is controlled by similar regulatory sequences. Plenum Publishing Corporation, 1990 Bunnell Bruce Department of Microbiology, University of Alabama at Birmingham, 35294, Birmingham, Alabama aut Fillmore Helen Department Cell Biology and Anatomy, University of Alabama at Birmingham, 35294, Birmingham, Alabama aut Gregory Paula Department Cell Biology and Anatomy, University of Alabama at Birmingham, 35294, Birmingham, Alabama aut Kidd Vincent Department of Microbiology, University of Alabama at Birmingham, 35294, Birmingham, Alabama aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/2(1990-03-01), 151-162 0740-7750 16:2<151 1990 16 11188 https://doi.org/10.1007/BF01233045 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01233045 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bunnell Bruce Department of Microbiology, University of Alabama at Birmingham, 35294, Birmingham, Alabama aut NATIONALLICENCE 700 1- Fillmore Helen Department Cell Biology and Anatomy, University of Alabama at Birmingham, 35294, Birmingham, Alabama aut NATIONALLICENCE 700 1- Gregory Paula Department Cell Biology and Anatomy, University of Alabama at Birmingham, 35294, Birmingham, Alabama aut NATIONALLICENCE 700 1- Kidd Vincent Department of Microbiology, University of Alabama at Birmingham, 35294, Birmingham, Alabama aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/2(1990-03-01), 151-162 0740-7750 16:2<151 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer