caa a22 4500 465820840 CHVBK 20180323112135.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF01233046 doi (NATIONALLICENCE)springer-10.1007/BF01233046 Generation of a panel of somatic cell hybrids containing unselected fragments of human chromosome 10 by X-ray irradiation and cell fusion: Application to isolating the MEN2A region in hybrid cells [Elektronische Daten] [P. Goodfellow, S. Povey, H. Nevanlinna, P. Goodfellow] We have used X-ray irradiation and cell fusion to generate somatic cell hybrids containing fragments of human chromosome 10. Our experiments were directed towards isolating the region of theMEN2A gene in hybrids and to use those as the source of DNA for cloning and mapping new markers from near theMEN2A locus. A number of hybrid clones containing human sequences that are tightly linked to theMEN2A gene were identified. Some 25% of our hybrids, however, proved to contain more than one human chromosome 10-derived fragment or showed evidence of deletions and/or rearrangements. A detailed analysis of the human content of X-ray irradiation hybrids is required to assess the integrity and number of human fragments retained. Despite retention of multiple human-derived fragments, these hybrids will prove useful as cloning and mapping resources. Plenum Publishing Corporation, 1990 Goodfellow P. Department of Medical Genetics, University of British Columbia, V6T 1W5, Vancouver, B.C., Canada aut Povey S. MRC Human Biochemical Genetics Unit, University College London, NW1 2HE, London, United Kingdom aut Nevanlinna H. Department of Obstetrics and Gynaecology, Helsinki University Hospital, 00290, Helsinkin, Finland aut Goodfellow P. Laboratory of Human Molecular Genetics, Imperial Cancer Research Fund, WC2A 3PX, London, United Kingdom aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/2(1990-03-01), 163-171 0740-7750 16:2<163 1990 16 11188 https://doi.org/10.1007/BF01233046 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01233046 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Goodfellow P. Department of Medical Genetics, University of British Columbia, V6T 1W5, Vancouver, B.C., Canada aut NATIONALLICENCE 700 1- Povey S. MRC Human Biochemical Genetics Unit, University College London, NW1 2HE, London, United Kingdom aut NATIONALLICENCE 700 1- Nevanlinna H. Department of Obstetrics and Gynaecology, Helsinki University Hospital, 00290, Helsinkin, Finland aut NATIONALLICENCE 700 1- Goodfellow P. Laboratory of Human Molecular Genetics, Imperial Cancer Research Fund, WC2A 3PX, London, United Kingdom aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/2(1990-03-01), 163-171 0740-7750 16:2<163 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer