caa a22 4500 465820859 CHVBK 20180323112135.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF01233042 doi (NATIONALLICENCE)springer-10.1007/BF01233042 Microdissection and microcloning of human chromosome 7q22-32 region [Elektronische Daten] [J. Weber, A. Weith, R. Kaiser, K. Grzeschik, K. Olek] Genetic information contributing to cystic fibrosis in addition on the CF gene is suggested to reside on the long arm of the human chromosome 7. In our attempt to analyze this genomic region in detail, we generated a region-specific DNA probe library by microdissection and microcloning of the midpiece of the chromosome 7q arm. Microdissection was performed in unstained metaphase spreads from a human × mouse hybrid cell line containing chromosome 7 as the only human chromosome. We obtained 593 clones from 75 dissected chromosomal fragments. At least 88% of the microclones were true recombinants; 40% of the clones contained repetitive sequences as determined by plaque hybridization with genomic DNA as probe. The overall mean fragment size of insert fragments was 3.2 kb, the median size was 3.5 kb. Regional mapping of 30 DNA fragments was performed by the aid of hybrid cell lines containing different segments of human chromosome 7; 50% of the microcloned inserts were found to map to 7q22-32. Plenum Publishing Corporation, 1990 Weber J. Institut für Klinische Biochemie der Universität Bonn, Sigmund-Freud-Str. 25, 5300, Bonn aut Weith A. Institut für experimentelle Pathologie, DKFZ Heidelberg, Im Neuenheimer Feld 280, 6900, Heidelberg aut Kaiser R. Institut für Klinische Biochemie der Universität Bonn, Sigmund-Freud-Str. 25, 5300, Bonn aut Grzeschik K. Institut für Humangenetik der Universität Marburg, Bahnhofstr. 7a, 3550, Marburg aut Olek K. Institut für Klinische Biochemie der Universität Bonn, Sigmund-Freud-Str. 25, 5300, Bonn aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/2(1990-03-01), 123-128 0740-7750 16:2<123 1990 16 11188 https://doi.org/10.1007/BF01233042 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01233042 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Weber J. Institut für Klinische Biochemie der Universität Bonn, Sigmund-Freud-Str. 25, 5300, Bonn aut NATIONALLICENCE 700 1- Weith A. Institut für experimentelle Pathologie, DKFZ Heidelberg, Im Neuenheimer Feld 280, 6900, Heidelberg aut NATIONALLICENCE 700 1- Kaiser R. Institut für Klinische Biochemie der Universität Bonn, Sigmund-Freud-Str. 25, 5300, Bonn aut NATIONALLICENCE 700 1- Grzeschik K. Institut für Humangenetik der Universität Marburg, Bahnhofstr. 7a, 3550, Marburg aut NATIONALLICENCE 700 1- Olek K. Institut für Klinische Biochemie der Universität Bonn, Sigmund-Freud-Str. 25, 5300, Bonn aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/2(1990-03-01), 123-128 0740-7750 16:2<123 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer