caa a22 4500 465820867 CHVBK 20180323112135.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF01233043 doi (NATIONALLICENCE)springer-10.1007/BF01233043 GC-rich murine adenosine deaminase gene promoter supports diverse tissue-specific gene expression [Elektronische Daten] [Sikha Rauth, Keh-Gang Yang, Anita Seibold, Diane Ingolia, Susan Ross, Cho-Yau Yeung] The murine adenosine deaminase (ADA) gene has a GC-rich promoter that is structurally typical of many mammalian "housekeeping” gene promoters. The ability of theADA gene promoter to support diverse tissue-specific gene expression was investigated EndogenousADA gene expression in different mouse tissues was found to vary over a >3000-fold range in a highly complex pattern. This range of expression was also observed in cultured human cell lines derived from different tissues. The ADA levels in all tissues and cell lines examined correlated closely with steady-state ADA mRNA levels. Several of the mouse tissues examined also showed stage-specific variation during postnatal development. In order to determine whether tissue-specificADA expression was controlled by cis-acting sequences upstream of the coding region, constructs containing a reporter gene regulated by the ADA gene's 5′ flanking sequences were used to generate transgenic mice. All transgene-expressing mice obtained showed diverse reporter gene expression in the tissues analyzed. Our results demonstrate that both in vivo and in the context of an integrated transgene this GC-rich promoter can support highly diverse gene expression in all tissues of the animal. Plenum Publishing Corporation, 1990 Rauth Sikha Department of Genetics, University of Illinois College of Medicine, 808 South Wood Street, 60612, Chicago, Illinois aut Yang Keh-Gang Department of Genetics, University of Illinois College of Medicine, 808 South Wood Street, 60612, Chicago, Illinois aut Seibold Anita Department of Biological Chemistry, University of Illinois College of Medicine, 808 South Wood Street, 60612, Chicago, Illinois aut Ingolia Diane Laboratory of Experimental Oncology, Department of Pathology, Stanford University School of Medicine, 94305, Stanford, California aut Ross Susan Department of Biological Chemistry, University of Illinois College of Medicine, 808 South Wood Street, 60612, Chicago, Illinois aut Yeung Cho-Yau Department of Genetics, University of Illinois College of Medicine, 808 South Wood Street, 60612, Chicago, Illinois aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/2(1990-03-01), 129-141 0740-7750 16:2<129 1990 16 11188 https://doi.org/10.1007/BF01233043 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01233043 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rauth Sikha Department of Genetics, University of Illinois College of Medicine, 808 South Wood Street, 60612, Chicago, Illinois aut NATIONALLICENCE 700 1- Yang Keh-Gang Department of Genetics, University of Illinois College of Medicine, 808 South Wood Street, 60612, Chicago, Illinois aut NATIONALLICENCE 700 1- Seibold Anita Department of Biological Chemistry, University of Illinois College of Medicine, 808 South Wood Street, 60612, Chicago, Illinois aut NATIONALLICENCE 700 1- Ingolia Diane Laboratory of Experimental Oncology, Department of Pathology, Stanford University School of Medicine, 94305, Stanford, California aut NATIONALLICENCE 700 1- Ross Susan Department of Biological Chemistry, University of Illinois College of Medicine, 808 South Wood Street, 60612, Chicago, Illinois aut NATIONALLICENCE 700 1- Yeung Cho-Yau Department of Genetics, University of Illinois College of Medicine, 808 South Wood Street, 60612, Chicago, Illinois aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/2(1990-03-01), 129-141 0740-7750 16:2<129 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer