caa a22 4500 465820875 CHVBK 20180323112135.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF01233040 doi (NATIONALLICENCE)springer-10.1007/BF01233040 Intragenic interspecific complementation of glucose 6-phosphate dehydrogenase in human-hamster cell hybrids [Elektronische Daten] [M. Town, M. Athanasiou-Metaxa, L. Luzzatto] A new variant of human glucose 6-phosphate dehydrogenase (G6PD), provisionally designated G6PD Harilaou, was observed in a Greek boy affected by severe hemolytic anemia. G6PD Harilaou was associated with very severe deficiency of enzyme activity, which measured about 1% of normal in the patient's fibroblasts. By fusion of Harilaou fibroblasts with a similarly enzyme-deficient mutant CHO cell line, we have isolated a hybrid cell line that has a G6PD activity 5-10 times higher than either of the parental cells. By electrophoretic analysis we show that most of this activity is associated with a hybrid dimeric G6PD molecule consisting of one hamster and one human subunit. We suggest that this heterologous quasi-interallelic complementation is effected by a catalytically abnormal hamster subunit stabilizing a catalytically abnormal and unstable Harilaou subunit. This approach may be useful for the study of dimer formation and stability in human G6PD. Plenum Publishing Corporation, 1990 Town M. Department of Haematology, Royal Postgraduate Medical School, Hammersmith Hospital, W12 ONN, London, United Kingdom aut Athanasiou-Metaxa M. First Paediatric Clinic, University Of Thessaloniki, Thessaloniki, Greece aut Luzzatto L. Department of Haematology, Royal Postgraduate Medical School, Hammersmith Hospital, W12 ONN, London, United Kingdom aut Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/2(1990-03-01), 97-108 0740-7750 16:2<97 1990 16 11188 https://doi.org/10.1007/BF01233040 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01233040 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Town M. Department of Haematology, Royal Postgraduate Medical School, Hammersmith Hospital, W12 ONN, London, United Kingdom aut NATIONALLICENCE 700 1- Athanasiou-Metaxa M. First Paediatric Clinic, University Of Thessaloniki, Thessaloniki, Greece aut NATIONALLICENCE 700 1- Luzzatto L. Department of Haematology, Royal Postgraduate Medical School, Hammersmith Hospital, W12 ONN, London, United Kingdom aut NATIONALLICENCE 773 0- Somatic Cell and Molecular Genetics Kluwer Academic Publishers-Plenum Publishers 16/2(1990-03-01), 97-108 0740-7750 16:2<97 1990 16 11188 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer