caa a22 4500 465822061 CHVBK 20180323112138.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF00164744 doi (NATIONALLICENCE)springer-10.1007/BF00164744 A thermostable alkaline β-1,3-glucanase produced by alkalophilic Bacillus sp. AG-430 [Elektronische Daten] [Yuichi Nogi, Koki Horikoshi] Summary: Alkalophilic Bacillus sp. AG-430 was isolated from soil and found to produce an extracellular β-1,3-glucanase. This enzyme was purified by chromatography on DEAE-sepharose CL-6B, Sephadex G-75 and hydroxyapatite. The enzyme was extremely thermostable and lost only 10% of the original activity after incubation at 100°C for 10 min. The optimum temperature and pH for activity were 60°-65°C and 9-10, respectively. The molecular weight was estimated at about 35 000 on sodium dodecyl sulphate-polyacrylamide gel eletrophoresis and the pI was about 3.8. The enzyme hydrolysed laminaritetraose, but not laminaritriose, and the end-products detected in the hydrolysate were identified as glucose, laminaribiose and laminaritriose. The enzyme split laminarin at random and yielded glucose, laminaribiose, laminaritriose and higher oligosaccharides. The enzyme is a type of endo-β-1,3-glucanase. Springer-Verlag, 1990 Nogi Yuichi The Superbugs Project, Research Development Corporation of Japan, c/o, The Riken Institute, 2-1, Hirosawa, 351-01, Wako, Saitama, Japan aut Horikoshi Koki Laboratory of Applied Bacteriology, The Riken Institute, 2-1, Hirosawa, 351-01, Wako, Saitama, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 32/6(1990-03-01), 704-707 0175-7598 32:6<704 1990 32 253 https://doi.org/10.1007/BF00164744 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164744 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nogi Yuichi The Superbugs Project, Research Development Corporation of Japan, c/o, The Riken Institute, 2-1, Hirosawa, 351-01, Wako, Saitama, Japan aut NATIONALLICENCE 700 1- Horikoshi Koki Laboratory of Applied Bacteriology, The Riken Institute, 2-1, Hirosawa, 351-01, Wako, Saitama, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/6(1990-03-01), 704-707 0175-7598 32:6<704 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer