caa a22 4500 465822088 CHVBK 20180323112138.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF00164729 doi (NATIONALLICENCE)springer-10.1007/BF00164729 d -Ribulokinase from Klebsiella pneumoniae for continuous production of d -(−)-ribulose-5-phosphate [Elektronische Daten] [Daniel Gygax, Henk Nachtegaal, Oreste Ghisalba, René Lattmann, Hans-Peter Schär, Christian Wandrey, Markus Streiff] Summary: The production of d-ribulose-5-phosphate in an enzyme membrane reactor was examined. Phosphoryl transfer from ATP to d-ribulose was catalysed by d-ribulokinase isolated from Klebsiella pneumoniae. For production of d-ribulose-5-phosphate the phosphoryl donor ATP was used either in stoichiometric or in catalytic amounts. Using catalytic amounts of ATP requires a second enzyme, e.g. pyruvate kinase, to regenerate ATP. The kinetic parameters for d-ribulokinase and pyruvate kinase were determined to calculate the performance of an enzyme membrane reactor for continuous production of d-ribulose-5-phosphate. Both processes operated for more than 200 h. Regardless of whether ATP was used in catalytic or stoichiometric amounts, about the same production parameters were determined. In continuous production space/time yields of 117 g (with ATP regeneration) and 103 g (without ATP regeneration) of d-ribulose-5-phosphate 1−1 per day were reached. Springer-Verlag, 1990 Gygax Daniel Central Research Laboratories of Ciba-Geigy Lrd., CH-4002, Basel, Switzerland aut Nachtegaal Henk Central Research Laboratories of Ciba-Geigy Lrd., CH-4002, Basel, Switzerland aut Ghisalba Oreste Central Research Laboratories of Ciba-Geigy Lrd., CH-4002, Basel, Switzerland aut Lattmann René Central Research Laboratories of Ciba-Geigy Lrd., CH-4002, Basel, Switzerland aut Schär Hans-Peter Central Research Laboratories of Ciba-Geigy Lrd., CH-4002, Basel, Switzerland aut Wandrey Christian Institut für Biotechnologie der KFA Jülich, D-5170, Jülich, Federal Republic of Germany aut Streiff Markus Central Research Laboratories of Ciba-Geigy Lrd., CH-4002, Basel, Switzerland aut Applied Microbiology and Biotechnology Springer-Verlag 32/6(1990-03-01), 621-626 0175-7598 32:6<621 1990 32 253 https://doi.org/10.1007/BF00164729 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164729 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gygax Daniel Central Research Laboratories of Ciba-Geigy Lrd., CH-4002, Basel, Switzerland aut NATIONALLICENCE 700 1- Nachtegaal Henk Central Research Laboratories of Ciba-Geigy Lrd., CH-4002, Basel, Switzerland aut NATIONALLICENCE 700 1- Ghisalba Oreste Central Research Laboratories of Ciba-Geigy Lrd., CH-4002, Basel, Switzerland aut NATIONALLICENCE 700 1- Lattmann René Central Research Laboratories of Ciba-Geigy Lrd., CH-4002, Basel, Switzerland aut NATIONALLICENCE 700 1- Schär Hans-Peter Central Research Laboratories of Ciba-Geigy Lrd., CH-4002, Basel, Switzerland aut NATIONALLICENCE 700 1- Wandrey Christian Institut für Biotechnologie der KFA Jülich, D-5170, Jülich, Federal Republic of Germany aut NATIONALLICENCE 700 1- Streiff Markus Central Research Laboratories of Ciba-Geigy Lrd., CH-4002, Basel, Switzerland aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/6(1990-03-01), 621-626 0175-7598 32:6<621 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer