caa a22 4500 465822096 CHVBK 20180323112138.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF00164749 doi (NATIONALLICENCE)springer-10.1007/BF00164749 Production of a herbicide, 5-aminolevulinic acid, by Rhodobacter sphaeroides using the effluent of swine waste from an anaerobic digestor [Elektronische Daten] [Ken Sasaki, Tohru Tanaka, Yoshinori Nishizawa, Mitsunori Hayashi] Summary: For the production of a herbicide, 5-amino-levulinic acid (ALA), from anaerobic digestion liquor, the utilization of the photosynthetic bacterium, Rhodobacter sphaeroides was examined. This bacterium could produce ALA extracelularly from this liquor with the addition of levulinic acid (LA), an inhibitor of ALA dehydratase (ALAD), and glycine, a precursor of ALA biosynthesis in the Shemin pathway. Succinate (another precursor) addition was unnecessary for ALA production. When repeated additions of LA were made together with glycine ALA production was significantly enhanced. However, above three additions of LA, ALA production was not further enhanced. The maximum value of ALA production attained was 4.2 mM (0.63 g/ 1), which was over double that of other ALA producers such as Chlorella vulgaris. Propionic acid was predominantly utilized compared with other lower fatty acids, suggesting that this might be converted to ALA via succinyl-coenzyme A (CoA) in the methylmalonyl-CoA pathway. Springer-Verlag, 1990 Sasaki Ken Hiroshima-Denki Institute of Technology, 20-1 Nakano, Akiku, 739-03, Hiroshima, Japan aut Tanaka Tohru Cosmo Research Institute, 1134-2 Gongendo, 340-01, Satte City Saitama, Japan aut Nishizawa Yoshinori Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Saijo-cho, Higashi-Hiroshima, 724, Hiroshima, Japan aut Hayashi Mitsunori Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Saijo-cho, Higashi-Hiroshima, 724, Hiroshima, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 32/6(1990-03-01), 727-731 0175-7598 32:6<727 1990 32 253 https://doi.org/10.1007/BF00164749 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164749 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sasaki Ken Hiroshima-Denki Institute of Technology, 20-1 Nakano, Akiku, 739-03, Hiroshima, Japan aut NATIONALLICENCE 700 1- Tanaka Tohru Cosmo Research Institute, 1134-2 Gongendo, 340-01, Satte City Saitama, Japan aut NATIONALLICENCE 700 1- Nishizawa Yoshinori Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Saijo-cho, Higashi-Hiroshima, 724, Hiroshima, Japan aut NATIONALLICENCE 700 1- Hayashi Mitsunori Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Saijo-cho, Higashi-Hiroshima, 724, Hiroshima, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/6(1990-03-01), 727-731 0175-7598 32:6<727 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer