caa a22 4500 46582210X CHVBK 20180323112138.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF00164735 doi (NATIONALLICENCE)springer-10.1007/BF00164735 Synthesis of 9-( β - d -arabinofuranosyl)guanine using whole cells of Escherichia coli [Elektronische Daten] [A. Zinchenko, V. Barai, S. Bokut, E. Kvasyuk, I. Mikhailopulo] Summary: Synthesis of 9-(β-d-arabinofuranosyl)guanine (ara-G) from 1-(β-d-arabinofuranosyl)cytosine (ara-C) and guanine, guanosine or 2′-deoxyguanosine (dG) by glutaraldehyde-treated Escherichia coli BM-11 cells is described. It is shown that the concentration of phosphate ions, molar ratio of substrates and pH of the reaction medium are factors affecting product yield. Under optimum conditions ara-G was produced in the reaction mixture in a yield of 63%-65% based on dG as the best source of guanine base. The yield of isolated ara-G was 48%-53%. Springer-Verlag, 1990 Zinchenko A. Institute of Microbiology, Byelorussian SSR Academy of Sciences, Zhodinskaya 2, 220733, Minsk, USSR aut Barai V. Institute of Microbiology, Byelorussian SSR Academy of Sciences, Zhodinskaya 2, 220733, Minsk, USSR aut Bokut S. Institute of Microbiology, Byelorussian SSR Academy of Sciences, Zhodinskaya 2, 220733, Minsk, USSR aut Kvasyuk E. Institute of Bio-Organic Chemistry, Byelorussian SSR Academy of Sciences, Minsk, USSR aut Mikhailopulo I. Institute of Bio-Organic Chemistry, Byelorussian SSR Academy of Sciences, Minsk, USSR aut Applied Microbiology and Biotechnology Springer-Verlag 32/6(1990-03-01), 658-661 0175-7598 32:6<658 1990 32 253 https://doi.org/10.1007/BF00164735 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164735 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zinchenko A. Institute of Microbiology, Byelorussian SSR Academy of Sciences, Zhodinskaya 2, 220733, Minsk, USSR aut NATIONALLICENCE 700 1- Barai V. Institute of Microbiology, Byelorussian SSR Academy of Sciences, Zhodinskaya 2, 220733, Minsk, USSR aut NATIONALLICENCE 700 1- Bokut S. Institute of Microbiology, Byelorussian SSR Academy of Sciences, Zhodinskaya 2, 220733, Minsk, USSR aut NATIONALLICENCE 700 1- Kvasyuk E. Institute of Bio-Organic Chemistry, Byelorussian SSR Academy of Sciences, Minsk, USSR aut NATIONALLICENCE 700 1- Mikhailopulo I. Institute of Bio-Organic Chemistry, Byelorussian SSR Academy of Sciences, Minsk, USSR aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/6(1990-03-01), 658-661 0175-7598 32:6<658 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer