caa a22 4500 465822134 CHVBK 20180323112138.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF00164741 doi (NATIONALLICENCE)springer-10.1007/BF00164741 Overproduction and single-step purification of Bacillus stearothermophilus peroxidase in Escherichia coli [Elektronische Daten] [Suvit Loprasert, Itaru Urabe, Hirosuke Okada] Summary: The cloned peroxidase gene from Bacillus stearothermophilus was highly expressed in Escherichia coli. Using the high copy number plasmid which is temperature-sensitive and its own strong promoter, this thermostable peroxidase was produced at 28% of the total cell proteins when the cells were grown at 42°C. The enzyme could be easily purified from E. coli by heat treatment and single-column Sephadex G-200 chromatography. From a 200 ml culture, 30 mg of purified enzyme was obtained. The peroxidase produced by E. coli showed a thermostability, haem type and content identical with those of the peroxidase produced by B. stearothermophilus. Springer-Verlag, 1990 Loprasert Suvit Department of Fermentation Technology, Faculty of Engineering, Osaka University, Yamada-oka, 565, Suita-shi, Osaka, Japan aut Urabe Itaru Department of Fermentation Technology, Faculty of Engineering, Osaka University, Yamada-oka, 565, Suita-shi, Osaka, Japan aut Okada Hirosuke Department of Fermentation Technology, Faculty of Engineering, Osaka University, Yamada-oka, 565, Suita-shi, Osaka, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 32/6(1990-03-01), 690-692 0175-7598 32:6<690 1990 32 253 https://doi.org/10.1007/BF00164741 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164741 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Loprasert Suvit Department of Fermentation Technology, Faculty of Engineering, Osaka University, Yamada-oka, 565, Suita-shi, Osaka, Japan aut NATIONALLICENCE 700 1- Urabe Itaru Department of Fermentation Technology, Faculty of Engineering, Osaka University, Yamada-oka, 565, Suita-shi, Osaka, Japan aut NATIONALLICENCE 700 1- Okada Hirosuke Department of Fermentation Technology, Faculty of Engineering, Osaka University, Yamada-oka, 565, Suita-shi, Osaka, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/6(1990-03-01), 690-692 0175-7598 32:6<690 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer