caa a22 4500 465822142 CHVBK 20180323112138.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF00164733 doi (NATIONALLICENCE)springer-10.1007/BF00164733 Glycosylation of ergot alkaloids by free and immobilized cells of Claviceps purpurea [Elektronische Daten] [Vladimír Křen, Miroslav Flieger, Přemysl Sajdl] Summary: A new strain, Claviceps purpurea 88-EP-47, with high invertase activity was selected. Free and Calginate immobilized cultures of this strain were used for fructosylation of ergot alkaloids. By bioconversion from their aglycones, elymoclavine-O-β-d-fructofuranosyl(2→1)-O-β-d-fructofuranoside, and elymoclavine-O-β-d-fructoside, the respective fructosides of chanoclavine, lysergol and dihydro-lysergol monofructosides were obtained. These substances are formed by β-d-fructofuranosidase present in Claviceps cells. The bioconversion activity of the enzyme system (fructose transfer) is strongly dependent on pH, substrate (sucrose) concentration and the developmental profile of invertase activity. The pH optimum for elymoclavine fructosylation is 6.5, for chanoclavine 5.7, and the optimal sucrose concentration is 75 g/l. Fifteen-day-old production cultures had the best glycosylation activities. Fructosylation of alkaloids can be stimulated in production cultures of C. purpurea or C. fusiformis forming elymoclavine or chanoclavine by a pH shift to 6.5 at the end of the production phase. Glycosylating Claviceps strains producing elymoclavine eliminate the free alkaloid into glycosides. The feedback inhibition of alkaloid synthesis by elymoclavine is then strongly reduced, helping to further improve elymoclavine yields. Elymoclavine can be liberated simply by invertase activity of baker's yeast. Springer-Verlag, 1990 Křen Vladimír Institute of Microbiology, Department of Biogenesis of Natural Substances, Czechoslovak Academy of Sciences, Vídeňská 1083, 14220, Prague 4, Czechoslovakia aut Flieger Miroslav Institute of Microbiology, Department of Biogenesis of Natural Substances, Czechoslovak Academy of Sciences, Vídeňská 1083, 14220, Prague 4, Czechoslovakia aut Sajdl Přemysl Institute of Microbiology, Department of Biogenesis of Natural Substances, Czechoslovak Academy of Sciences, Vídeňská 1083, 14220, Prague 4, Czechoslovakia aut Applied Microbiology and Biotechnology Springer-Verlag 32/6(1990-03-01), 645-650 0175-7598 32:6<645 1990 32 253 https://doi.org/10.1007/BF00164733 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164733 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Křen Vladimír Institute of Microbiology, Department of Biogenesis of Natural Substances, Czechoslovak Academy of Sciences, Vídeňská 1083, 14220, Prague 4, Czechoslovakia aut NATIONALLICENCE 700 1- Flieger Miroslav Institute of Microbiology, Department of Biogenesis of Natural Substances, Czechoslovak Academy of Sciences, Vídeňská 1083, 14220, Prague 4, Czechoslovakia aut NATIONALLICENCE 700 1- Sajdl Přemysl Institute of Microbiology, Department of Biogenesis of Natural Substances, Czechoslovak Academy of Sciences, Vídeňská 1083, 14220, Prague 4, Czechoslovakia aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/6(1990-03-01), 645-650 0175-7598 32:6<645 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer