caa a22 4500 465822207 CHVBK 20180323112138.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF00164746 doi (NATIONALLICENCE)springer-10.1007/BF00164746 Stimulation by cysteine on growth of Clostridium thermoaceticum in minimal medium [Elektronische Daten] [Aneth Koesnandar, Naomichi Nishio, Shiro Nagai] Summary: An acetate-tolerant strain of Clostridium thermoaceticum (ATCC 31490), characterized as growing on a tryptone-yeast extract complex medium, was adapted to grow in a minimal medium containing glucose as sole carbon source. After five sequential batch cultures involving reducing and deleting organic materials from the original complex medium, the bacterium could grow on minimal medium with a supplement of nicotinic acid. However, growth and homoacetogenesis from glucose was remarkably retarded compared with that observed in the original complex medium. This could be strikingly improved up to the original level by replacing sodium thioglycolate (a reducing agent in the original medium) with L-cysteinλHCI supplemented to the minimal medium. Cysteine played an important role in stimulating growth and homoacetogenesis by not only supplying H2S as a sulphur source via cysteine desulphydrase (EC 4.4.1.1) but also maintaining a proper redox potential (—370 and —400 mV) in the growth phase during culture. Methionine could not serve as a sulphur source. Springer-Verlag, 1990 Koesnandar Aneth Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Higashi Hiroshima 724, Saijo-cho, Japan aut Nishio Naomichi Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Higashi Hiroshima 724, Saijo-cho, Japan aut Nagai Shiro Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Higashi Hiroshima 724, Saijo-cho, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 32/6(1990-03-01), 711-714 0175-7598 32:6<711 1990 32 253 https://doi.org/10.1007/BF00164746 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164746 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koesnandar Aneth Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Higashi Hiroshima 724, Saijo-cho, Japan aut NATIONALLICENCE 700 1- Nishio Naomichi Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Higashi Hiroshima 724, Saijo-cho, Japan aut NATIONALLICENCE 700 1- Nagai Shiro Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Higashi Hiroshima 724, Saijo-cho, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/6(1990-03-01), 711-714 0175-7598 32:6<711 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer