caa a22 4500 465822215 CHVBK 20180323112138.0 cr unu---uuuuu 170327e19900301xx s 000 0 eng 10.1007/BF00164740 doi (NATIONALLICENCE)springer-10.1007/BF00164740 Expression of an Erwinia sp. gene encoding diphenyl ether cleavage in Escherichia coli and an isolated Acinetobacter strain PE7 [Elektronische Daten] [Hungming Liaw, V. Srinivasan] Summary: An Acinetobacter strain PE7 with the ability to grow on salicylic acid and to degrade diphenyl ethers was isolated from a petroleum waste pit in Louisiana. A cloned Erwinia sp. dpe gene encoding diphenyl ether cleavage was introduced into PE7 in order to enhance its degradative ability. A broad-host-range expression plasmid, pDPE2388, was constructed by inserting an SspI-HpaI fragment from a dpe gene-containing plasmid, pDPE7321, into the kanamycin resistance gene of plasmid pKT230. The DNA fragment contained the dpe gene flanked between sp6 and T7 promoters. Transconjugants of pDPE2388 plasmid into PE7 were isolated. Expression of the dpe gene in Escherichia coli or PE7 displayed a degradative ability to cleave the following diphenyl ethers: 4-chlorodiphenyl ether, 4-nitrodiphenyl ether, and 4-hydroxydiphenyl ether. Springer-Verlag, 1990 Liaw Hungming Department of Microbiology, Louisiana State University, 70803, Baton Rouge, LA, USA aut Srinivasan V. Department of Microbiology, Louisiana State University, 70803, Baton Rouge, LA, USA aut Applied Microbiology and Biotechnology Springer-Verlag 32/6(1990-03-01), 686-689 0175-7598 32:6<686 1990 32 253 https://doi.org/10.1007/BF00164740 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164740 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liaw Hungming Department of Microbiology, Louisiana State University, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Srinivasan V. Department of Microbiology, Louisiana State University, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/6(1990-03-01), 686-689 0175-7598 32:6<686 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer