caa a22 4500 465822258 CHVBK 20180323112138.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00903768 doi (NATIONALLICENCE)springer-10.1007/BF00903768 Continuous microbial production of l -leucine with cell retention [Elektronische Daten] [Rolf Wichmann, Christian Wandreg, Joachim Große-Wiesmann] Summary: Continuous production ofl-leucine was carried out withCorynebacterium glutamicum, strain ATCC 13032 starting fromα-ketoisocaproic acid as the precursor, glucose as the carbon source and ammonium sulphate as the nitrogen source, with biotin in a mineral medium. By means of cross-flow microfiltration or centrifugal separation for cell retention in continuous fermentation an increase in cell density was achieved and the product solution was obtained cell-free. The cells were concentrated to over 70 g cell dry mass/1. In experiments of up to 42 days, conversion rates of 85%-99% andl-leucine yields of 85%-93% were achieved. With a substrate residence time of 3.6 h, 114 mmol/1l-leucine was produced with a space-time yield of 97 g/1 per day. A scale-up of the fermentation volume from 4 to 1001 provided comparable results. Springer-Verlag, 1990 Wichmann Rolf Arbeitsgruppe Bioverfahrenstechnik, Fachbereich Chemietechnik, Universität Dortmund, Postfach 500500, D-4600, Dortmund 50, Germany aut Wandreg Christian Institut für Biotechnologie der Kernforschungsanlage Jülich GmbH, Postfach 1913, D-5170, Jülich, Germany aut Große-Wiesmann Joachim Degussa AG, FCO-B, Kantstrasse 2, D-4802, Halle-Künsebeck, Germany aut Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 373-379 0175-7598 32:4<373 1990 32 253 https://doi.org/10.1007/BF00903768 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00903768 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wichmann Rolf Arbeitsgruppe Bioverfahrenstechnik, Fachbereich Chemietechnik, Universität Dortmund, Postfach 500500, D-4600, Dortmund 50, Germany aut NATIONALLICENCE 700 1- Wandreg Christian Institut für Biotechnologie der Kernforschungsanlage Jülich GmbH, Postfach 1913, D-5170, Jülich, Germany aut NATIONALLICENCE 700 1- Große-Wiesmann Joachim Degussa AG, FCO-B, Kantstrasse 2, D-4802, Halle-Künsebeck, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 373-379 0175-7598 32:4<373 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer