caa a22 4500 465822266 CHVBK 20180323112138.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00903776 doi (NATIONALLICENCE)springer-10.1007/BF00903776 Diffusion in immobilized-cell agar layers: influence of microbial burden and cell morphology on the diffusion coefficients of l -malic acid and glucose [Elektronische Daten] [Laurent Mignot, Guy-Alain Junter] Summary: The diffusivities ofl-malic acid and glucose in an agar membrane entrapping small amounts ofEscherichia coli orRhodospirillum rubrum whole cells were measured using time lag (TL) and steady state (SS) methods. Diffusivities were overestimated by the SS method. For concentrations of immobilizedR. rubrum cells ranging between 104 and 109 organisms cm−3 agar (20 ng-2 mg dry weight cm−3 agar), the diffusion coefficient ofl-malic acid, determined by both methods, was related to the logarithm of the membrane cell content by a decreasing linear relationship. The diffusion coefficient of glucose obtained by TL analysis was not significantly affected by the presence in the membrane of 3 ng-0.3 mg dry wt.E. coli cm−3 agar. However, values arising from the SS method decreased linearly as a function of the amount of immobilized organisms. Membranes containingR. rubrum cells offered higher diffusional resistance tol-malic acid and glucose than those loaded with the same amount ofE. coli cells. Springer-Verlag, 1990 Mignot Laurent Laboratory of Macromolecular Chemistry, University of Rouen, URA CNRS No. 500, PO Box 118, 76134, Mont-Saint-Aignan cédex, France aut Junter Guy-Alain Laboratory of Macromolecular Chemistry, University of Rouen, URA CNRS No. 500, PO Box 118, 76134, Mont-Saint-Aignan cédex, France aut Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 418-423 0175-7598 32:4<418 1990 32 253 https://doi.org/10.1007/BF00903776 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00903776 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mignot Laurent Laboratory of Macromolecular Chemistry, University of Rouen, URA CNRS No. 500, PO Box 118, 76134, Mont-Saint-Aignan cédex, France aut NATIONALLICENCE 700 1- Junter Guy-Alain Laboratory of Macromolecular Chemistry, University of Rouen, URA CNRS No. 500, PO Box 118, 76134, Mont-Saint-Aignan cédex, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 418-423 0175-7598 32:4<418 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer