caa a22 4500 465822304 CHVBK 20180323112138.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00903787 doi (NATIONALLICENCE)springer-10.1007/BF00903787 Degradation of phenanthrene, fluorene and fluoranthene by pure bacterial cultures [Elektronische Daten] [Walter Weissenfels, Michael Beyer, Jürgen Klein] Summary: Bacterial mixed cultures able to degrade the polycyclic aromatic hydrocarbons (PAH) phenanthrene, fluorene and fluoranthene, were obtained from soil using conventional enrichment techniques. From these mixed cultures three pure strains were isolated:Pseudomonas paucimobilis degrading phenanthrene;P. vesicularis degrading fluorene andAlcaligenes denitrificans degrading fluoranthene. The maximum rates of PAH degradation ranged from 1.0 mg phenanthrene/ml per day to 0.3 mg fluoranthene/ml per day at doubling times of 12 h to 35 h for growth on PAH as sole carbon source. The protein yield during PAH degradation was about 0.25 mg/mg C for all strains. Maximum PAH oxidation rates and optimum specific bacterial growth were obtained near pH 7.0 and 30°C. After growth entered the stationary phase, no dead end-products of PAH degradation could be detected in the culture fluid. Springer-Verlag, 1990 Weissenfels Walter Bergbau-Forschung GmbH, Abteilung Entsorgungstechnik C-EA 1, Franz Fischer Weg 61, D-4300, Essen 13, Germany aut Beyer Michael Bergbau-Forschung GmbH, Abteilung Entsorgungstechnik C-EA 1, Franz Fischer Weg 61, D-4300, Essen 13, Germany aut Klein Jürgen Bergbau-Forschung GmbH, Abteilung Entsorgungstechnik C-EA 1, Franz Fischer Weg 61, D-4300, Essen 13, Germany aut Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 479-484 0175-7598 32:4<479 1990 32 253 https://doi.org/10.1007/BF00903787 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00903787 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Weissenfels Walter Bergbau-Forschung GmbH, Abteilung Entsorgungstechnik C-EA 1, Franz Fischer Weg 61, D-4300, Essen 13, Germany aut NATIONALLICENCE 700 1- Beyer Michael Bergbau-Forschung GmbH, Abteilung Entsorgungstechnik C-EA 1, Franz Fischer Weg 61, D-4300, Essen 13, Germany aut NATIONALLICENCE 700 1- Klein Jürgen Bergbau-Forschung GmbH, Abteilung Entsorgungstechnik C-EA 1, Franz Fischer Weg 61, D-4300, Essen 13, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 479-484 0175-7598 32:4<479 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer