caa a22 4500 465822320 CHVBK 20180323112138.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00903777 doi (NATIONALLICENCE)springer-10.1007/BF00903777 Genetic complementation of Streptomyces tendae deficient in nikkomycin production [Elektronische Daten] [Christiane Bormann, Karin Aberle, Hans-P. Fiedler, Hildgund Schrempf] Summary: Streptomyces tendae Tü 901 produces the nucleoside peptide antibiotic nikkomycin. In shot-gun cloning experiments using pIJ699 as vector we isolated a 9.4-kb DNA fragment fromS. tendae which complemented the nikkomycin nonproducing mutant NP9 to the formation of nikkomycin C/Cx and Kx. Nikkomycins were identified by HPLC analyses and their characteristic UV spectra. In Southern hybridization experiments the cloned DNA exclusively reacted withS. tendae DNA sequences. As shown by Northern dot blotting, transcripts of the isolated DNA fragment were only detected during stationary growth and correlated with the extent of nikkomycin production. When the recombinant plasmid pNP113 containing the 9.4-kb DNA fragment was transferred into the over-producing mutant Tü901/ S2566, transformants exhibited a significantly decreased capacity for forming nikkomycin. Southern analysis of genomic DNA of these transformants revealed that severe rearrangements occurred in DNA sequences homologous to the 9.4-kb insert of pNP113. Springer-Verlag, 1990 Bormann Christiane Universität Tübingen, Biologie II, Auf der Morgenstelle 28, D-7400, Tübingen, Germany aut Aberle Karin Universität Tübingen, Biologie II, Auf der Morgenstelle 28, D-7400, Tübingen, Germany aut Fiedler Hans-P Universität Tübingen, Biologie II, Auf der Morgenstelle 28, D-7400, Tübingen, Germany aut Schrempf Hildgund Universität München, Institut für Genetik und Mikrobiologie, Maria Ward Strasse la, D-8000, Munich 19, Germany aut Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 424-430 0175-7598 32:4<424 1990 32 253 https://doi.org/10.1007/BF00903777 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00903777 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bormann Christiane Universität Tübingen, Biologie II, Auf der Morgenstelle 28, D-7400, Tübingen, Germany aut NATIONALLICENCE 700 1- Aberle Karin Universität Tübingen, Biologie II, Auf der Morgenstelle 28, D-7400, Tübingen, Germany aut NATIONALLICENCE 700 1- Fiedler Hans-P Universität Tübingen, Biologie II, Auf der Morgenstelle 28, D-7400, Tübingen, Germany aut NATIONALLICENCE 700 1- Schrempf Hildgund Universität München, Institut für Genetik und Mikrobiologie, Maria Ward Strasse la, D-8000, Munich 19, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 424-430 0175-7598 32:4<424 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer