caa a22 4500 465822355 CHVBK 20180323112139.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00903779 doi (NATIONALLICENCE)springer-10.1007/BF00903779 Screening of basidiomycetes for lignin peroxidase genes using a DNA probe [Elektronische Daten] [Yoshio Kimura, Yasuhiko Asada, Masaaki Kuwahara] Summary: Basidiomycetes were screened for lignin peroxidase (LPO) genes using a DNA probe prepared from the LPO restriction fragment ofPhanerochaete chrysosporium. Southern blot analysis showed restriction fragments of chromosomal DNA ofBjerkandera adusta andCoriolus consors hybridized with the probe.Bjerkandera adusta produced LPO in a glucose-peptone medium. Ion-exchange chromatography showed that this fungus produced multiple molecular forms of LPO. One of the enzymes, LPO-2, was purified and characterized. The molecular weight of LPO-2 was 41000 with a pI of 4.2. Spectral analysis demonstrated that LPO-2 is a haem protein. The enzyme cleaved lignin model dimers mainly at the Cα-Cβ position of the side chain. The LPO-2 exhibited close similarity to LPOs ofP. chrysosporium with respect to their basic properties. Springer-Verlag, 1990 Kimura Yoshio Department of Bioresource Science, Kagawa University, Miki-Cho, 761-07, Kagawa, Japan aut Asada Yasuhiko Department of Bioresource Science, Kagawa University, Miki-Cho, 761-07, Kagawa, Japan aut Kuwahara Masaaki Department of Bioresource Science, Kagawa University, Miki-Cho, 761-07, Kagawa, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 436-442 0175-7598 32:4<436 1990 32 253 https://doi.org/10.1007/BF00903779 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00903779 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kimura Yoshio Department of Bioresource Science, Kagawa University, Miki-Cho, 761-07, Kagawa, Japan aut NATIONALLICENCE 700 1- Asada Yasuhiko Department of Bioresource Science, Kagawa University, Miki-Cho, 761-07, Kagawa, Japan aut NATIONALLICENCE 700 1- Kuwahara Masaaki Department of Bioresource Science, Kagawa University, Miki-Cho, 761-07, Kagawa, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 436-442 0175-7598 32:4<436 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer