caa a22 4500 465822363 CHVBK 20180323112139.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00903786 doi (NATIONALLICENCE)springer-10.1007/BF00903786 Fages Jacques Pioneer France-Maïs, Seed Microbiology Research Laboratory, Chemin de l'enseigure, F-31840, Aussonne, France aut An optimized process for manufacturing an Azospirillum inoculant for crops [Elektronische Daten] [Jacques Fages] Summary: An optimized process for manufacturing a crop moculant was developed with anAzospirillum lipoferum strain. This process involves the entrapment of living cells in alginate beads and dehydration. The influence of several parameters, alginate concentration, additions of adjuvants at different stages, dilution of culture broth, water activity and dehydration method on bacterial survival is presented. The highest survival was obtained by addition of skim milk and controlled air-dehydration of the alginate beads. Finally, a powdered inoculant was obtained, containing more than 10 billion cells/g, easy to store and to handle, which can be used in the field as a microgranule or as a seed coating. Furthermore, the biodegradability insures that there is no environmental pollution. Springer-Verlag, 1990 Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 473-478 0175-7598 32:4<473 1990 32 253 https://doi.org/10.1007/BF00903786 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00903786 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Fages Jacques Pioneer France-Maïs, Seed Microbiology Research Laboratory, Chemin de l'enseigure, F-31840, Aussonne, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 473-478 0175-7598 32:4<473 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer