caa a22 4500 46582238X CHVBK 20180323112139.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00903784 doi (NATIONALLICENCE)springer-10.1007/BF00903784 Production of hydrogen peroxide by aryl-alcohol oxidase from the ligninolytic fungus Pleurotus eryngii [Elektronische Daten] [Francisco Guillén, Angel Martinez, Maria Martínez] Summary: Production of extracellular hydrogen peroxide by fungal oxidases is been investigated as a requirement for lignin degradation. Aryl-alcohol oxidase activity is described in extracellular liquid and mycelium ofPleurotus eryngii and studied under non-limiting nitrogen conditions. This aryl-alcohol oxidase catalyses conversion of primary aromatic alcohols to the corresponding aldehydes and H2O2, showing no activity with aliphatic and secondary aromatic alcohols. The enzyme is stable at pH 4.0-9.0, has maximal activity at 45°-50°C and pH 6.0-6.5, is inhibited by Ag+, Pb2+ and NaN3, and has aK m of 1.2 mM using veratryl alcohol as substrate. A single protein band with aryl-alcohol oxidase activity was found in zymograms of extracellular and intracellular crude enzyme preparations fromP. eryngii. Springer-Verlag, 1990 Guillén Francisco Unidad de Microbiologia Aplicada, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Velázquez 144, 28006, Madrid, Spain aut Martinez Angel Unidad de Microbiologia Aplicada, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Velázquez 144, 28006, Madrid, Spain aut Martínez Maria Unidad de Microbiologia Aplicada, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Velázquez 144, 28006, Madrid, Spain aut Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 465-469 0175-7598 32:4<465 1990 32 253 https://doi.org/10.1007/BF00903784 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00903784 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guillén Francisco Unidad de Microbiologia Aplicada, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Velázquez 144, 28006, Madrid, Spain aut NATIONALLICENCE 700 1- Martinez Angel Unidad de Microbiologia Aplicada, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Velázquez 144, 28006, Madrid, Spain aut NATIONALLICENCE 700 1- Martínez Maria Unidad de Microbiologia Aplicada, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Velázquez 144, 28006, Madrid, Spain aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 465-469 0175-7598 32:4<465 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer