caa a22 4500 465822401 CHVBK 20180323112139.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00903781 doi (NATIONALLICENCE)springer-10.1007/BF00903781 Nutritional control of actinorhodin production by Streptomyces coelicolor A3(2): suppressive effects of nitrogen and phosphate [Elektronische Daten] [Janice Doull, Leo Vining] Summary: Actinorhodin production inStreptomyces coelicolor A3(2) was relatively insensitive to the carbon source concentration but was elicited by nitrogen or phosphate depletion, or by a decline in the growth rate. In starch-glutamate media with nitrogen limitation, increasing the nitrogen supply delayed the onset of antibiotic synthesis and, at concentrations above 30 mM, decreased its rate. In a similar medium with phosphate limitation, increasing the initial phosphate concentration delayed actinorhodin formation and, above 2.5 mM, reduced the rate of synthesis. Experiments in which actinorhodin synthesis was elicited by phosphate depletion at various nitrogen concentrations demonstrated strong suppression by residual glutamate. Cultures in which actinorhodin biosynthesis was initiated by nitrogen depletion were not similarly suppressed by increasing amounts of residual phosphate. The results suggest that actinorhodin production inS. coelicolor A3(2) responds to interacting physiological controls, notable among which is nitrogen catabolite regulation. Springer-Verlag, 1990 Doull Janice Biology Department, Dalhousie University, B3H 4J1, Halifax, Nova Scotia, Canada aut Vining Leo Biology Department, Dalhousie University, B3H 4J1, Halifax, Nova Scotia, Canada aut Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 449-454 0175-7598 32:4<449 1990 32 253 https://doi.org/10.1007/BF00903781 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00903781 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Doull Janice Biology Department, Dalhousie University, B3H 4J1, Halifax, Nova Scotia, Canada aut NATIONALLICENCE 700 1- Vining Leo Biology Department, Dalhousie University, B3H 4J1, Halifax, Nova Scotia, Canada aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 449-454 0175-7598 32:4<449 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer