caa a22 4500 46582241X CHVBK 20180323112139.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00903780 doi (NATIONALLICENCE)springer-10.1007/BF00903780 Cloning of a DNA fragment from Corynebacterium glutamicum conferring aminoethyl cysteine resistance and feedback resistance to aspartokinase [Elektronische Daten] [Georg Thierbach, Jörn Kalinowski, Bernd Bachmann, Alfred Pühler] Summary: TheCorynebacterium glutamicum/Escherichia coli shuttle vector plasmid pZ1 was used to clone the S-(2-aminoethyl)-d,l-cysteine (AEC)-resistance gene from a lysine-excreting, AEC-resistant strain ofC. glutamicum, the aspartokinase activity of which was released from feedback inhibition by mixtures of lysine and threonine or AEC and threonine respectively. A recombinant plasmid designated pCS2 carrying a 9.9-kb chromosomal insert that conferred AEC resistance and the ability to excrete lysine to its host was isolated. The aspartokinase activity of the pCS2-carrying strain was resistant towards inhibition by mixtures of lysine and threonine or AEC and threonine respectively. By deletion analysis the DNA region conferring AEC resistance to the host and feedback resistance to its aspartokinase activity could be confined to a 1.2-kb DNA fragment. Springer-Verlag, 1990 Thierbach Georg Degussa AG/ASTA Pharma AG, Kantstrasse 2, D-4802, Bielefeld 1, Germany aut Kalinowski Jörn Fakultät für Biologie VI, Universität Bielefeld, Postfach 8640, D-4800, Bielefeld 1, Germany aut Bachmann Bernd Degussa AG/ASTA Pharma AG, Kantstrasse 2, D-4802, Bielefeld 1, Germany aut Pühler Alfred Fakultät für Biologie VI, Universität Bielefeld, Postfach 8640, D-4800, Bielefeld 1, Germany aut Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 443-448 0175-7598 32:4<443 1990 32 253 https://doi.org/10.1007/BF00903780 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00903780 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thierbach Georg Degussa AG/ASTA Pharma AG, Kantstrasse 2, D-4802, Bielefeld 1, Germany aut NATIONALLICENCE 700 1- Kalinowski Jörn Fakultät für Biologie VI, Universität Bielefeld, Postfach 8640, D-4800, Bielefeld 1, Germany aut NATIONALLICENCE 700 1- Bachmann Bernd Degussa AG/ASTA Pharma AG, Kantstrasse 2, D-4802, Bielefeld 1, Germany aut NATIONALLICENCE 700 1- Pühler Alfred Fakultät für Biologie VI, Universität Bielefeld, Postfach 8640, D-4800, Bielefeld 1, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 443-448 0175-7598 32:4<443 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer