caa a22 4500 465822452 CHVBK 20180323112139.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00903769 doi (NATIONALLICENCE)springer-10.1007/BF00903769 Production of glycerol by immobilized Pichia farinosa [Elektronische Daten] [B. Bisping, U. Baumann, H. Rehm] Summary: Cells of the osmophilic yeastPichia farinosa were immobilized in sintered glass Raschig rings for the production of glycerol. The kinetics of production were observed under different conditions in batch, fed-batch and semicontinuous fermentations in fixed-bed column reactors and compared with those of free cells. 2.6 × 109 cells/g sintered glass were adsorbed. The glycerol productivity amounted to 8.1 g/l per day. The highest concentration reached in batch culture was 86 g/l with immobilized cells. Fermentations using immobilized cells were accelerated compared to fermentations using free cells and maximum yield and productivity were reached at lower initial sugar concentrations. Using scanning electron microscopy it was observed that the shape of the cells was related to the sugar concentration in the medium. The experiments show thatP. farinosa produces glycerol with a high and constant productivity over long periods of time. Springer-Verlag, 1990 Bisping B. Institut für Mikrobiologie, Universität Münster, Corrensstrasse 3, D-4400, Münster, Germany aut Baumann U. Institut für Mikrobiologie, Universität Münster, Corrensstrasse 3, D-4400, Münster, Germany aut Rehm H. Institut für Mikrobiologie, Universität Münster, Corrensstrasse 3, D-4400, Münster, Germany aut Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 380-386 0175-7598 32:4<380 1990 32 253 https://doi.org/10.1007/BF00903769 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00903769 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bisping B. Institut für Mikrobiologie, Universität Münster, Corrensstrasse 3, D-4400, Münster, Germany aut NATIONALLICENCE 700 1- Baumann U. Institut für Mikrobiologie, Universität Münster, Corrensstrasse 3, D-4400, Münster, Germany aut NATIONALLICENCE 700 1- Rehm H. Institut für Mikrobiologie, Universität Münster, Corrensstrasse 3, D-4400, Münster, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/4(1990-01-01), 380-386 0175-7598 32:4<380 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer