caa a22 4500 465822495 CHVBK 20180323112139.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00173725 doi (NATIONALLICENCE)springer-10.1007/BF00173725 Oriented immobilization of bacterial photosynthetic membrane [Elektronische Daten] [Masayuki Hara, Toshikazu Majima, Jun Miyake, Shu-ichi Ajiki, Hiroaki Sugino, Hideki Toyotama, Sugio Kawamura] Summary: We have examined a method for oriented immobilization of photosynthetic membrane fragments on a solid surface by specific avidin-biotin interaction. Photosynthetic membrane fragments from the purple non-sulphur photosynthetic bacterium Rhodopseudomonas viridis, of which the H-subunit of the photosynthetic reaction centre was biotinylated, was immobilized on an avidin-adsorbed plate. Orientation of the immobilized membrane on the plastic plate was checked by an antisera binding assay that could react to the respective sides of the membrane: the H-subunit side was selectively adsorbed on the plate. Light-induced potential and current responses could be measured when the membrane immobilized on the SnO2-coated glass plate was dried and sandwiched with a counter electrode of Hg. The electrical response in the immobilized membrane was much improved in comparison with the control (membranes were simply adsorbed on the plate), supporting the idea that the membranes have an orientation on solid surfaces. Springer-Verlag, 1990 Hara Masayuki Fermentation Research Institute, Agency of Industrial Science and Technology (AIST), Ministry of International Trade and Industry (MITI), 1-1-3 Higashi, Tsukuba, 305, Ibaraki, Japan aut Majima Toshikazu Electrotechnical Laboratory, AIST, MITI, 1-1-4 Umezono, Tsukuba, 305, Ibaraki, Japan aut Miyake Jun Fermentation Research Institute, Agency of Industrial Science and Technology (AIST), Ministry of International Trade and Industry (MITI), 1-1-3 Higashi, Tsukuba, 305, Ibaraki, Japan aut Ajiki Shu-ichi Tsukuba Research Laboratory, Stanley Electric Co., Ltd., 5-9-5 Tokodai, Tsukuba, 300-26, Ibaraki, Japan aut Sugino Hiroaki Tsukuba Research Laboratory, Stanley Electric Co., Ltd., 5-9-5 Tokodai, Tsukuba, 300-26, Ibaraki, Japan aut Toyotama Hideki Tsukuba Research Laboratory, Stanley Electric Co., Ltd., 5-9-5 Tokodai, Tsukuba, 300-26, Ibaraki, Japan aut Kawamura Sugio Fermentation Research Institute, Agency of Industrial Science and Technology (AIST), Ministry of International Trade and Industry (MITI), 1-1-3 Higashi, Tsukuba, 305, Ibaraki, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 32/5(1990-02-01), 544-549 0175-7598 32:5<544 1990 32 253 https://doi.org/10.1007/BF00173725 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00173725 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hara Masayuki Fermentation Research Institute, Agency of Industrial Science and Technology (AIST), Ministry of International Trade and Industry (MITI), 1-1-3 Higashi, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Majima Toshikazu Electrotechnical Laboratory, AIST, MITI, 1-1-4 Umezono, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Miyake Jun Fermentation Research Institute, Agency of Industrial Science and Technology (AIST), Ministry of International Trade and Industry (MITI), 1-1-3 Higashi, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Ajiki Shu-ichi Tsukuba Research Laboratory, Stanley Electric Co., Ltd., 5-9-5 Tokodai, Tsukuba, 300-26, Ibaraki, Japan aut NATIONALLICENCE 700 1- Sugino Hiroaki Tsukuba Research Laboratory, Stanley Electric Co., Ltd., 5-9-5 Tokodai, Tsukuba, 300-26, Ibaraki, Japan aut NATIONALLICENCE 700 1- Toyotama Hideki Tsukuba Research Laboratory, Stanley Electric Co., Ltd., 5-9-5 Tokodai, Tsukuba, 300-26, Ibaraki, Japan aut NATIONALLICENCE 700 1- Kawamura Sugio Fermentation Research Institute, Agency of Industrial Science and Technology (AIST), Ministry of International Trade and Industry (MITI), 1-1-3 Higashi, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/5(1990-02-01), 544-549 0175-7598 32:5<544 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer