caa a22 4500 465822525 CHVBK 20180323112139.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00173728 doi (NATIONALLICENCE)springer-10.1007/BF00173728 Characterization and complementation of a cephalosporin-deficient mutant of Streptomyces clavuligerus NRRL 3585 [Elektronische Daten] [Jacqueline Piret, Bertha Resendiz, Bernd Mahro, Jin-You Zhang, Eugene Serpe, Jorge Romero, Neal Connors, Arnold Demain] Summary: We have characterized a mutant of Streptomyces clavuligerus NRRL 3585 which is almost completely blocked in cephalosporin biosynthesis and exhibits depressed activities of both the delta(l-alpha-aminoadipyl)-l-cysteinyl-d-valine (ACV) synthetase and cyclase enzymes of the cephalosporin pathway. A wild-type DNA region was cloned which partially restores antibiotic production, ACV synthetase and cyclase activities to this mutant. The recombinant plasmid exhibits a variable copy number in different transformants. Hybridization experiments indicate that sequences homologous to the cloned region are present in various β-lactam-producing Streptomyces spp. but absent in species which are not known to produce this class of antibiotics. Furthermore, the chromosomal copy of the cloned region lies in close proximity to a gene coding for the isopenicilin N synthase gene of the cephalosphorin pathway. Springer-Verlag, 1990 Piret Jacqueline Department of Biology, Northeastern University, 02115, Boston, MA, USA aut Resendiz Bertha Department of Biology, Northeastern University, 02115, Boston, MA, USA aut Mahro Bernd Department of Biology, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA aut Zhang Jin-You Department of Biology, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA aut Serpe Eugene Department of Biology, Northeastern University, 02115, Boston, MA, USA aut Romero Jorge Department of Biology, Northeastern University, 02115, Boston, MA, USA aut Connors Neal Department of Biology, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA aut Demain Arnold Department of Biology, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA aut Applied Microbiology and Biotechnology Springer-Verlag 32/5(1990-02-01), 560-567 0175-7598 32:5<560 1990 32 253 https://doi.org/10.1007/BF00173728 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00173728 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Piret Jacqueline Department of Biology, Northeastern University, 02115, Boston, MA, USA aut NATIONALLICENCE 700 1- Resendiz Bertha Department of Biology, Northeastern University, 02115, Boston, MA, USA aut NATIONALLICENCE 700 1- Mahro Bernd Department of Biology, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA aut NATIONALLICENCE 700 1- Zhang Jin-You Department of Biology, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA aut NATIONALLICENCE 700 1- Serpe Eugene Department of Biology, Northeastern University, 02115, Boston, MA, USA aut NATIONALLICENCE 700 1- Romero Jorge Department of Biology, Northeastern University, 02115, Boston, MA, USA aut NATIONALLICENCE 700 1- Connors Neal Department of Biology, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA aut NATIONALLICENCE 700 1- Demain Arnold Department of Biology, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/5(1990-02-01), 560-567 0175-7598 32:5<560 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer