caa a22 4500 465822606 CHVBK 20180323112139.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00173727 doi (NATIONALLICENCE)springer-10.1007/BF00173727 Mechanism of the stimulatory effect of cyclodextrins on lankacidin-producing Streptomyces [Elektronische Daten] [Hidekazu Sawada, Takashi Suzuki, Shun-ichi Akiyama, Yoshio Nakao] Summary: Gel-filtration analysis of a mixture of cyclodextrin (CyD) and lankacidin C showed that β-CyD had strong, γ-CyD weak and α-CyD no affinity for lankacidin C. Lankacidin C production activity, which was assayed by measuring the incorporation of l-[methyl-14C-]methionine into the lankacidin molecule, was the greatest with cells grown in the presence of β-CyD, less with γ-CyD and the least with α-CyD. Lankamycin and T-2636M, which are by-products in lankacidin C fermentation, were not included by β-CyD and their production was not stimulated by β-CyD. It was apparent that the stimulatory effect of CyD was closely related to the formation of an inclusion complex between CyD and the antibiotic. Lankacidin C biosynthesis was repressed by preincubating cells with lankacidin C, while the repressive effect of lankacidin C was abrogated by the inclusion by β-CyD. Thus, abrogation of feed-back repression seems to be a main mechanism of the effect of CyD. However, α-CyD, which had no affinity for lankacidin C, stimulated the production to the least extent and exhibited a complementary effect on the stimulation by β-CyD or γ-CyD. α-CyD also caused a change in cell morphology and cell-surface hydrophobicity. It was assumed that the modification of the cell surface is a secondary mechanism of the effect of CyD. Springer-Verlag, 1990 Sawada Hidekazu Microbiology Research Laboratories, Research and Development Division, Takeda Chemical Industries, Ltd., Yodogawa-ku, 532, Osaka, Japan aut Suzuki Takashi Microbiology Research Laboratories, Research and Development Division, Takeda Chemical Industries, Ltd., Yodogawa-ku, 532, Osaka, Japan aut Akiyama Shun-ichi Microbiology Research Laboratories, Research and Development Division, Takeda Chemical Industries, Ltd., Yodogawa-ku, 532, Osaka, Japan aut Nakao Yoshio Microbiology Research Laboratories, Research and Development Division, Takeda Chemical Industries, Ltd., Yodogawa-ku, 532, Osaka, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 32/5(1990-02-01), 556-559 0175-7598 32:5<556 1990 32 253 https://doi.org/10.1007/BF00173727 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00173727 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sawada Hidekazu Microbiology Research Laboratories, Research and Development Division, Takeda Chemical Industries, Ltd., Yodogawa-ku, 532, Osaka, Japan aut NATIONALLICENCE 700 1- Suzuki Takashi Microbiology Research Laboratories, Research and Development Division, Takeda Chemical Industries, Ltd., Yodogawa-ku, 532, Osaka, Japan aut NATIONALLICENCE 700 1- Akiyama Shun-ichi Microbiology Research Laboratories, Research and Development Division, Takeda Chemical Industries, Ltd., Yodogawa-ku, 532, Osaka, Japan aut NATIONALLICENCE 700 1- Nakao Yoshio Microbiology Research Laboratories, Research and Development Division, Takeda Chemical Industries, Ltd., Yodogawa-ku, 532, Osaka, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/5(1990-02-01), 556-559 0175-7598 32:5<556 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer