caa a22 4500 465822614 CHVBK 20180323112139.0 cr unu---uuuuu 170327e19900201xx s 000 0 eng 10.1007/BF00173721 doi (NATIONALLICENCE)springer-10.1007/BF00173721 Emulsifying agents from bacteria isolated during screening for cells with hydrophobic surfaces [Elektronische Daten] [Thomas Neu, Karl Poralla] Summary: The culture supernatants of 126 bacterial strains isolated during screening for hydrophobic cell surfaces, were tested for the production of emulsifying agents. Forty-eight strains were found to produce effective emulsion-stabilizing substances during growth on glucose. The most effective emulsifying agents were isolated and could be divided into two chemical groups. The first group was separated from the isolated extracts by the use of thin-layer chromatography and detected as ninhydrin-negative, 4,4'-tetramethyldiamino-diphenylmethane-positive spots. The amino acid composition indicated surfactin and iturin, produced by one Bacillus species, and viscosin, produced by a Pseudomonas species. The second group was identified as polymeric substances. The chemical characterization of five polymers showed polysaccharides that were able to stabilize emulsions. From these the neutral and charged monosaccharides were determined qualitatively. The constituents of the five isolated polysaccharides were: strain 5, glucose, strain 17, rhamnose, glucose, glucuronic acid; strain 33, rhamnose, galactose, glucose. glucuronic acid; strain 113, fucose, galactose, glucose, galacturonic acid, glucosamine; strain 259, one unknown compound, rhamnose, galactose, glucuronic acid. Springer-Verlag, 1990 Neu Thomas Botanisches Institut, Mikrobiologie, Universität Tübingen, Auf der Morgenstelle 1, D-7400, Tübingen, Federal Republic of Germany aut Poralla Karl Botanisches Institut, Mikrobiologie, Universität Tübingen, Auf der Morgenstelle 1, D-7400, Tübingen, Federal Republic of Germany aut Applied Microbiology and Biotechnology Springer-Verlag 32/5(1990-02-01), 521-525 0175-7598 32:5<521 1990 32 253 https://doi.org/10.1007/BF00173721 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00173721 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Neu Thomas Botanisches Institut, Mikrobiologie, Universität Tübingen, Auf der Morgenstelle 1, D-7400, Tübingen, Federal Republic of Germany aut NATIONALLICENCE 700 1- Poralla Karl Botanisches Institut, Mikrobiologie, Universität Tübingen, Auf der Morgenstelle 1, D-7400, Tübingen, Federal Republic of Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 32/5(1990-02-01), 521-525 0175-7598 32:5<521 1990 32 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer